梅毒患者红细胞CR1密度相关基因多态性研究-皮肤病与性病学专业毕业论文.docxVIP

兰州大学硕．I：学位论文梅毒患者红细胞CRl密度相关基因多态性研究 兰州大学硕．I：学位论文 梅毒患者红细胞CRl密度相关基因多态性研究 摘 要 梅毒是由梅毒螺旋体引起的一种危害性极大的慢性传染病，主要通过性接触 和血液传播。梅毒的发病机制未完全阐明，出现血清固定的确切原因目前尚不清 楚。当前对梅毒发病机制和血清固定原因的研究主要集中在白细胞免疫，认为梅 毒发病与T细胞介导的免疫反应密切相关。近年来红细胞免疫逐渐成为感染性 疾病的研究热点之一，有研究表明梅毒感染者的红细胞免疫功能较正常人明显降 低，但关于梅毒患者CRl密度相关基因多态性的研究尚未见报道。 目的探讨梅毒患者红细胞CRl密度相关基因型的频率分布。 方法采用聚合酶链反应(PCR)、限制性内切酶HindIII酶切技术测定患者 红细胞CRl密度相关基因多态性。 结果 病例组中CRl基因HH、HL和LL基因型分布频率分别为58．75％、 35％、和6．25％，对照组中HH、HL和LL基因型分布频率分别为68．75％、26．25％ 和5％，两组CRl基因型的分布频率差异无显著性(pO．05)；梅毒患者组基因 突变率为41．25％，对照组为31．25％，也无显著性差异(p0．05)。 结论梅毒患者的红细胞免疫功能低下与红细胞CRl基因发生突变关系不 密切。 关键词梅毒；红细胞；1型补体受体；基因多态性 兰州人学硕J：学位论文A 兰州人学硕J：学位论文 A Study on CRl Gene Density Polymorphism of Erythrocyte in Patients with Syphilis Abstract Background Syphilis is a chronic infectious disease of great harmfulness caused by Treponema pallidum，mainly spread through sexual contact and blood．The pathogenesis of syphilis is not completely clear,and the exact cause of the serum fixed is not clear．At present studies of the pathogenesis of syphilis and the causes of serum fixed mainly focused on leucocyte immune，it is hold that the incidence of syphilis and T cell·mediated immune response are closely linked．In recent years， erythrocyte immune become one of the research hot spots of infectious diseases， studies have shown that erythrocyte immune functions in persons infected with Treponema pallidum were significantly reduced，but study of CRl gene density polymorphism on erythrocyte in patients with syphilis has not been reported． Objective To explore the erythrocyte CRl gene density polymorphism frequency in patients with syphilis． Methods CRl gene density polymorphism was measured using PCR amplification followed by Hind III restriction enzyme digestion． Results The percentages of HH，HL and LL genotypes were 58．75％，35％and 6．25％for syphilis patients，and 68．75％，26．25％and 5％for healthy controls， respectively．Statistical analysis showed that there Was no significant difference between the healthy controls and syphilis patients