罗格列酮对急性酒精性胃粘膜损伤的保护作用-急诊内科学专业毕业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 见黏膜层出血，血管严重充血；奥美拉唑组大鼠胃黏膜表层有部分上 皮细胞脱落，腺体结构大部分完整，较多的中性粒细胞浸润，充血明 显减轻；罗格列酮组大鼠胃黏膜部分上皮细胞脱落、破坏，胃腺体少 量深层坏死，可见中性粒细胞浸润，胃黏膜轻微充血；联合用药组大 鼠胃黏膜损伤仅仅局限于黏膜表层，部分上皮细胞脱落，腺体结构基 本完整，少量被破坏，可见到中性粒细胞浸润，胃黏膜仅轻微充血。 4．各组胃黏膜COX-2蛋白表达水平比较：正常对照组未见明显 COX-2阳性产物表达；损伤组及奥美拉唑组COX-2蛋白表达均明显 高于正常对照组，组间差异有统计学意义(P均(o．05，但两组问差 异没有统计学意义(P0．05)；罗格列酮组和联合用药组COX．2蛋 白表达水平均显著高于正常组，但低于损伤组和奥美拉唑组，组间差 异有统计学意义(P均O．05)，但罗格列酮组和联合用药组两组问表 达差异没有统计学意义(P0．05)。 结论 1．奥美拉唑对急性酒精性胃黏膜损伤有保护作用。 2．罗格列酮对酒精诱导的急性胃黏膜损伤有保护作用，但单独 应用其保护作用不及奥美拉唑的保护作用强。 3．罗格列酮与奥美拉唑联合应用对急性胃黏膜损伤的保护作用 最显著。 4．罗格列酮对酒精诱导的急性胃黏膜损伤保护作用的机制与其 对胃黏膜促炎症因子的抑制有关。 关键词罗格列酮，酒精，环氧化酶．2，过氧化物酶体增殖物激 活受体 U 硕士学位论文 硕士学位论文 英文摘要 OBJIECTIVE This study was designed to determine the protective effect of rosigiitazon，a specific ligand for peroxisome proliferators activated receptor gamma(PPAR-y)，on gastric mucosal lesions induced in rats by alcohol administration and it relation to eyclcoxygenase-2 (cox-2)． METIIOD 50 healthy Male S-D rats were employed in the study and divided into 5 groups randomly accord吨to weight．The rats fasted for 24 h before the study，rats received pretreatment：normal control group and injury model group(saline 5ml／kg i．g．)；omeprazol group(Omeprazol 30 mg／kg i．V．)；rosiglitazon group(resiglitazon 8 mg,／l【g i．g．)；co-treatment group(rosig]。itazon 8 mg／kg i．g．and Omeprazol 30 mg／kg i．v．)respectively．After 0．5 h，all the animals except normal control group were given administration of 56。Hongxing Erguotou wine(18ml／kg)．The same treatment Was carried out on normal control group except that physiological saline was applied instead．Rats were killed six hours after alcohol administration．．The index of gastric mucosal injury Was evaluated macroscopically according to Guth’S method．Gastric mucosa structural changes were abserved under