黄颡鱼小肠结肠炎耶尔森氏菌的鉴定及致病性研究-水生态学杂志.DOCVIP

PAGE PAGE 10 黄颡鱼小肠结肠炎耶尔森氏菌16SrDNA及系统发育分析 苟小兰1，王 利1*，黄艳青2 （1.西南民族大学 生命科学与技术学院 动物遗传育种学国家民委-教育部重点实验室，四川成都 610041 2. 中国水产科学研究院 东海水产研究所，上海，200090） 摘要：从发病患什么病？待鉴定黄颡鱼心脏中分离到1株细菌GM2402，对该菌株进行分离培养、形态学观察、生理生化试验与16S rDNA基因的PCR扩增及序列分析。结果表明，菌株GM2402为革兰氏阴性杆菌，具动力性，V-P试验、水杨酸、过氧化氢酶、脲酶等阳性，不发酵鼠李糖、乳糖等，硫化氢、枸椽酸盐等阴性。16S rDNA序列长1417bp，采用什么方法获得的此结果要说明。采用PCR方法进行体外扩增GenBank中的登录号是JX101598，且与数据库中小肠结肠炎耶尔森氏菌（Yersinia enterocolitica）的相似性高达99.6%，系统发育树显示与小肠结肠炎耶尔森氏菌聚为一个分支。综合菌株GM2402的生化特性及分子生物学分析结果，判定菌株GM2402为小肠结肠炎耶尔森氏菌,为水生动物致病菌的分子鉴定积累了参考资料。 患什么病？待鉴定 采用什么方法获得的此结果要说明。采用PCR方法进行体外扩增 关键词：黄颡鱼；小肠结肠炎耶尔森氏菌；16SrDNA；系统发育分析 Phylogenetic Analysis and 16S rDNA of Yersinia enterocolitica Isolated from Yellow Cartfish（Pelteobagrus fulvidraco）注明英文和拉丁文 注明英文和拉丁文 GOU Xiao-lan1, WANG Li1*, HUANG Yanqing2 (1.Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities , Sichuan Chengdu 610041 2.East?China?Sea?fisheries?Research?Institute，Chinese?Academy?of?Fishery?Sciences, Shanghai 200090) Abstract:英文摘要需要修改。 One bacteria strain named GM2402 was isolated from the heart of natural diseased yellow cartfish (Pelteobagrus fulvidraco), the strain was identified according to its morphological characteristics, physiology and biochemistry experiments, as well as, PCR amplification of 16S rDNA genes and sequence analysis. The results showed that strain GM2402 was \t Gram-negative \t bacterium, the ability to movement and MR-VP, salicylic acid, catalase and urease were positive, but L-rhamnose, Galactose, H2S and citrate salt were negative. 16S rDNA sequence of strain GM2402 was 1417bp and accession number was JX101598. Strain GM2402 shared 99.6% identity with Yersinia enterocolitica strains in GenBank database. Phylogenetic tree demonstrated that strain GM2402 was gathered into one cluster with Y. enterocolitica. Both biochemical character and molecular analysis were proved that strain GM2402 was Y.