毛竹提取物对醚菌酯的抑菌增效作用及制剂加工-农药学专业毕业论文.docxVIP

PAGE PAGE III Abstract In this paper, by orthogonal experimental design, the best condition for extraction of bamboo leaves was established, and the antifungal synergism of bamboo extract with kresoxim-methy to Gibberella sanbinetti was studied in the laboratory. The preparations of bamboo extract and the combination with kresoxim-methy were developed. The field control efficiencies of the selected preparations to pepper gray mold were evaluated. The main results were following: 95% ethanol was taken as extraction solvent, and a 20L multi-function extraction and enrichment unit was used as test equipment.The orthogonfl design were used to optimum for the best condition for extraction of bamboo leaves. The best extraction conditions was 70℃ as extracting temperature, 1.5 hours per time, two times of extraction and 1:25(W/V) of material and alcohol ratio. Under this condition, the extraction of bamboo extract optimal. The BE EW was developed using selection of solvents, emulsifiers and other additives.The formulations were:10% bamboo extact, 10% emulsifiers, 20% solvent, 4% antifreeze N, 0.2% thickener M, adding water to 100%. The experiments of thermal storage, cold storage, melt-freeze, the stability experiments and persistent foaming of BE EW were developed, and the results showed that there had no phenomenon of hierarchical agent, sedimentation, water separating performance and a small amount of oil separating. The characters of BE EW were in accordance with the quality sandards of EW preparation. The antifungal synergism of bamboo extract with kresoxim-methy to Gibberella sanbinetti Miura was studied in the laboratory. The results showed that the EC50 values of the mixtures with the ratios of 5:1, 7:1, 9:1, 15:1, 49:1 and 99:1 (bamboo extract: kresoxim-methyl) were 2.37×10-2，2.56×10-2，2.25×10-2，3.20×10-2，23.20×10-2，191.3×10-2, respectively, which were lower than the EC50 value of the bamboo extract (1.20×103mg/L) , and