人参不同单体皂苷对大肠杆菌的生物热动力学表达.DOCVIP

人参皂苷Rg1、Rb1、Rd对大肠杆菌生物热活性的影响 曹俊岭1，李宏1，范秀荣1，肖小河2?国家自然科学基金项目 ； ?国家自然科学基金项目 ；国家重点基础研究发展计划(973计划)：2007CB512607 1 北京中医药大学东直门医院药学部 北京100700 2 解放军302医院 全军中药研究所 北京100063 摘要 目的：探讨人参皂苷Rg1、Rb1、Rd对大肠杆菌生物热活性的差异，为中药的活性筛选构建新的方法。方法：应用生物热动力学的方法，对人参皂苷Rg1、Rb1和Rd对生物模型的热活性的影响进行研究，计算总体发热量（Q），最高发热量（Pmax），最高发热时间(Tmax)代谢速率常数1（k1）和代谢速率常数2（k2）等热力学参数，应用主成分分析等统计方法对数据进行处理，并应用琼脂扩散法对各种皂苷的抑菌活性进行验证。结果 人参皂苷Rg1、Rb1在小剂量时对大肠杆菌代谢产热有轻微的促进作用，但随着剂量的增加则作用下降且产生抑制作用,但二者没有明显的差别；人参皂苷Rd从小剂量时就可使Pmax降低，k2下降，并随着剂量的增加而增加（P0.01），且在同等剂量下，其降低Pmax、k2的作用明显强于人参皂苷Rg1、Rb1（P0.01），各种皂苷的常规抑菌活性与生物热活性结果基本一致。结论 人参皂苷Rd对大肠杆菌的代谢产热有较强的抑制作用，人参皂苷Rg1、Rb1次之；同时为生物热动力学方法在药效物质筛选方面的应用提供依据。 关键词 人参皂苷Rg1、Rb1、Rd 大肠杆菌 生物热活性 Effects of Biothermo-activity of 3 Genosides on Escherichia coli Cao Juning, Li Zulun, Xiao Xiaohe 1 Pharmacy Department, Dongzhimen Hospital Affiliate to Beijing University of TCM (Beijing 100700); 2 PLA Institute of Chinese Material Medica，302 Hospital of PLA (Beijing 100039 ); 3 College of Pharmacy, Chengdu University of TCM (Sichuan Abstract objective:To investigate the difference of biothermo-activity of 3 genosides on Escherichia coli and establish the method for activity screening of Chinese material medica. Method: Biothermokinetics was used to examine the effects of different genosides on Escherichia coli. Thermodynamics parameter, such as Q , Pmax, Tmax, k1 and k2 were calculated. And they were analysis by principal component analysis. Result: Genosides-Rg1 and Rb1 can promote the metabolic heat production of Escherichia coli in low dosages. But with increasing of dosage, the heat production of Escherichia coli is inhibited. The heat production of Escherichia coli is inhibited by Genosides -Rd. and it is superior to genosides -Rg1 and Rb1（P0.01）. Conclusions：Genosides -Rd has the best effects to inhibit the heat production of Escherichia coli and Ginsengside-Rg1 and Rb1 were inferior to it. Meanwhile, this lab also provide the evidences for the application of thermokinetics on activity scre