慢乙肝患者HBVcccDNA与血清HBVDNA及其标志物相关性分析-内科学专业毕业论文.docxVIP

宁夏医科大学硕士研究生学位论文摘要 宁夏医科大学硕士研究生学位论文 摘要 II II 结果、HBsAg 滴度、ALT、AST 水平均无相关。肝组织 HBVcccDNA 定量检测联合血清 HBVDNA 和 HBeAg 是判断抗病毒治疗疗效的可靠指标。 关键词：肝炎病毒，乙型， HBV 共价闭合环状 DNA，实时定量荧光 PCR，乙肝血清 标记物，肝组织病理 III III The first part The Correlation study between Liver tissue HBVcccDNA of patients with chronic hepatitis B and serum HBVDNA and markers ABSTRACT Objective To investigate the association among chronic viral hepatitis B (CHB) patients liver tissue HBVcccDNA ,serum HBV DNA and Serum hepatitis B markers. Methods Selected 10 cases of chronic viral hepatitis B patients as research subjects, using Real time fluorescent quantitative PCR to detect liver tissue HBVcccDNA, using PCR - Fluorescence Probing to detect serum HBV DNA, with electricity chemiluminescence immunoassay ration to detect hepatitis B markers, the routine pathological staining analyze of pathological changes in liver tissue inflammation grades (G) and the degree of fibrosis staging (S). Results ① 10 cases of CHB patients liver tissue pathology results: G2S1:3 cases; G2S2:2 cases; G2S3:1 cases; G3S2:2 cases; G3S3:2 case. ②All of the 10 cases of CHB patients liver tissue were detected HBVcccDNA, quantitative value ranged from 1.80 x104 copies/mL to 8.50x109 copies/mL. 10 cases of CHB patients serum HBV DNA: 8 cases were positive: quantitative value ranged from 2.23 xl05 copies/mL to 3.08xl08 copies/mL, the other two cases were negative (below detection limit 103 copies/mL). ③The quantitative of HBVcccDNA in liver tissue and liver tissue inflammation activity ( G ) and the degree of fibrosis ( S ) had no significant correlation（Ｐ0.05）.④ The liver tissue HBVcccDNA quantitative value has high correlation with serum HBV DNA (ｒ=0.898，Ｐ0.01). ⑤ There was a high correlation between liver tissue HBVcccDNA quantitative value and serum quantitative value of HBeAg(r=0.821, Ｐ0.01)，but no correlation between HBsAg(r=-0.483, PAGE PAGE VI Ｐ0.05）. ⑥The liver tissue HBVcccDNA quantitative value between serum q