铝胁迫对紫花苜蓿的影响及外源有机酸的缓解机制-草业科学专业毕业论文.docx

IV IV inhibited the growth of M. sativa (P 0.05). Above-ground biomass, below-ground biomass, root length, leaf Fv/Fm value and root vigor under the acid and Al3+ stress treatment were significantly lower than those under the control treatment (pH6.0, no Al3+ added) (P 0.05). Contrarily, leaf malondialdehyde content and peroxidase content under the acid and Al3+ stress treatment were significantly elevated (P 0.05). The acid and Al3+ stress treatment also altered the processes of nitrogen metabolism. Leaf alanine content, ammonia content and praline content were significantly elevated (P 0.05) under the acid and Al3+ stress treatment. Moreover, antagonism among metallic ions absorbed by M. sativa roots was found under the acid and Al3+ stress treatment. Al3+ content in M. sativa roots under the acid and Al3+ stress treatment was twice as high as that under the control treatment, while K+、Zn2+、Na+、Mn2+ and Mg2+ contents under the acid and Al3+ stress treatment were decreased by a large extent. 4. Spraying four acids (i.e. oxalic acid, malic acid, citric acid and succinic acid) significantly relieved Al3+ stress for M. sativa. Above-ground biomass, below-ground biomass and root length of M. sativa plants under the acid spraying treatment were significantly higher than those under the control treatment, and root vigor, leaf malondialdehyde content and peroxidase content were improved. The effects of antagonism among metallic ions absorbed by M. sativa roots were also relived. Of the four acids, the effects of spraying oxalic acid were the shortest in duration and lower in magnitude, and the effects of spraying citric acid and succinic acid were the longest in duration and most significant in magnitude. These results suggest that different cultivators of M. sativa differed significantly in their tolerances to Al3+ stress and that spraying organic acids could relieve Al3+ stress for M. sativa. These results may benefit to the breeding of M. sativa cultivators that ar