HB-EGF及TGF-β1在单、多支冠脉狭窄冠心病患者血液中表达的实验研究.docVIP

groups. Detected the serum level of HB-EGF and TGF -β1 of three groups by double-antibody sandwich ABC-ELISA method.Took blood of three groups, through extracting RNA, reversing transcription, PCR, agarose gel electrophoresis, observatiing the outcome by gel imaging analysis system,took photo of outcome and measuring optical density value of aim gene and the internal reference markers β -actin.Use the ratio as statistical parameters.Using SPSS13.0 software package to analyze The experimental data. Tested all groups of quantitative data groups with normality test and homogeneity test of variances.Between two groups were analysised by two independent samples T test, single factor analysis of variance, Pearson correlation analysis to analyze other possible related factors. With P 0.05 for the test level. Results: Level in blood of HB-EGF and HB-EGF mRNA were significantly higher in CHD group than NC group(P0.05); Level in blood of TGF-β1 and TGF-β1 mRNA were significantly lower in CHD group than NC group(P0.05); Level in blood of HB-EGF and HB-EGF mRNA were significantly higher in M group than S group(P0.05); Level in blood of TGF-β1 and TGF-β1 mRNA were significantly lower in M group than S group(P0.05); BMI was positively correlated with the expression of HB-EGF and HB-EGF mRNA , negatively correlatied with the expression of TGF-β1 and TGF-β1 mRNA. Conclusion: The HB-EGF level in the CHD group patients blood is higher than normal groups both in protein and in gene transcription，it has a positive correlation with the level of AS lesion severity and branchs of coronary artery stenosis. 6 万方数据 The TGF-β1 level in the CHD group patients blood is lower than normal groups both in protein and in gene transcription，it has a negative correlation with the level of AS lesion severity and branchs of coronary artery stenosis. Obesity maybe an independent risk factor of CHD. Keywords: HB-EGF; TGF-β1; CHD; single coronary artery stenosis; multivessel coronary artery stenosis 7 万