乳酸菌对免疫调节活性之机能性评价.DOCVIP

乳酸菌對免疫調節活性之機能性評估 Evaluation of Function of Immunomodulatory Activity for Lactic Acid Bateria 研究生：邱文瑩 Chiu, Wen-Ying 指導教授：謝寶全 Hsieh, Pao-Chuan 陳武元 Chen, Wu-Yuan 【摘要】 本研究收集市售商品及自然界樣品分離出生長快速之乳酸菌，測試評估其益生菌特性，並且以體外及體內試驗評估乳酸菌之免疫調控活性。在體外試驗結果顯示有21株乳酸菌具備有益生菌之基本特性，如對pH 3具有耐受性、生長於0.3%膽鹽中及可吸附於腸道細胞。由以上試驗結果，挑選出5株乳酸菌(B-121、123、 145、183 及184)，進一步與人類週邊血液單核細胞進行培養，結果顯示5株乳酸菌具有抑制PHA刺激之週邊血液單核細胞(PBMCs)增殖能力，不同菌株具有減少Th2 細胞激素(IL-4及IL-6)之分泌，以及增加IFN-γ、TNF-α及IL-10的產生。而B-121可以顯著增加IFN-γ和IL-10及減少IL-4產生，基於這些結果，本研究挑選出B-121菌株進行毒性評估。結果顯示，B-121菌株不會侵入腸道上皮細胞株Caco-2，且有增加初代小鼠肝細胞株(BNL CL.2)之存活率及抑制人類肝癌細胞株(Hep G2)之增殖。在體內試驗則使用以卵白蛋白誘發之小鼠氣喘模式，在抗原刺激前餵食B-121菌株，發現經B-121菌株處理之後，可以減緩細胞浸潤至氣管及肺部組織中，且降低Th2細胞激素產生及氣管過度反應之現象。綜合以上結果，推測乳酸菌B-121有免疫調節之機能性功效。 關鍵字：乳酸菌、過敏、細胞激素、免疫調節 【Abstract】 Isolated strains of LAB from commercial products and natural sources were assessed for probiotic properties such as bile resistance, acid tolerance, and adhesion to intestinal mucous in this study. The immunomodulatory activity of LAB evaluated in vitro and in vivo. In vitro results showed that there were 21 strains the basic requirements for probiotic functions as they demonstrated probiotic characteristics such as tolerance to pH 3, growth in 0.3% oxgall and adhesion to intestinal cell. The study selected 5 LAB strains (B-121, 123, 145, 183 and 184) that were tested with allergic peripheral blood mononuclear cell (PBMCs). The results showed that 5 LAB inhibited PHA-stimulated PBMCs proliferation. Different strains decreased the secretion of Th2 cytokines (IL-4 and IL-6) and increased high levels of IFN-γ, TNF-α, and IL-10. IL-10 production resulted in partial to suppression of Th2 cytokines production and concurred with an increase in pro-inflammatory cytokines as IFN-γ and TNF-α. And B-121 strain showed a significantly increase expression of IFN-γ and IL-10 and decreased production of the cytokine IL-4. Based on these results, we selected B-121 strain that were tested for toxicity evaluation. The results showed that B-