蓝果忍冬果实转录组测序及花青素合成关键基因的研究-园艺学；果树学专业毕业论文.docxVIP

CoNTENTSAbstract CoNTENTS Abstract in Chinese ··J Abstract in English 一III 1．IⅡtroduction ． 一I 1．1 Purpose and significance 1 1．2 National and abroad literature summarization ·1 1．2．1 Overview ofanthocyanins 1 I．2．2 Biosynthesis and regulation of anthocyanin ·z 1．2．3 Advances in sequencing technology ·j 1．2．4 Overview oftranscriptome ··o 1．2．5 Secondary metabolism ofbule honeysuckle． ．． ．． ．． ．． ·· ··· ······- 9 1．3 Research content -10 1．4 Technology roadmap ’1 2．MateriaIs and Methods ··12 2．1 Materials ·12 2．2 Methods． ． ． 一13 2．2．1 Anthocyanins species and content analysis ofblue honeysuckle fruit 13 2．2．2 Antioxidant activity determination ofblue honeysuckle fruit ． ．．13 2．2．3 Transcriptome sequencing ofblue honeysuckle fruit．．．． - 15 2．2．4 Laboratory instruments and data analysis software 19 3．Results analysis ． ’17 3．1 Anthocyanin analysis ofblue honeysuckle ·l了 3．1．1 Determine ofanthocyanins qualitatively · 19 3．1．2 Determination ofanthocyanin quantitatively ．． ．． ．．．．．．． · ····-·· ·- ··· 21 3．2 Analysis ofAntioxidant activity 一zz 3．3 Analysis oftranseriptome sequencing zj 3．3．1 Sequencing and assemble of short reads ·zj 3．3．2 Functional annotation ofAll·unigenes ·25 3．3．3 Different expression analysis ofAll．unigenes 29 3．3．4 Identification ofrelated genes involved in anthocyanin biosynthesis 31 3．4 Identification ofrelated genes in different periods ofblue honeysuckle fruit ·31 3．5 Identiffcation ofrelated genes in different species ofblue honeysuckle fruit ·32 4．Discussion ～3斗 4．1 Determination ofanthocyanins species and content ，斗 4．2 Antioxidant activity ofblue honeysuckle ． - ‘‘j斗 4．3 TranscriDtome sequencing ofblue honeysuckle fruit ·34 4．4 Anthocvanin biosynthesis analysis ofblue honeysuckle fruit ·35 IIl 万方数据 东北农业大学农学硕士学位论文5．Conclusion 东北农业大学农学硕士学位论文 5．Conclusion ．37 Acknowledgements ． ．38 References