昆虫病原线虫优良品系的筛选及生物学特性分析-植物保护；农业昆虫与害虫防治专业毕业论文.docxVIP

CONTENTSCoNTENTS CONTENTS CoNTENTS Abstract in Chinese I Abstract in English ．． ． ．． ． ．． ．． ．．．． ． ． ． ．．Ill l IntroductiOn 一l l。1 Summary of entomopathogenie nematodes 1 1．1．1 Entomopathogenic nematodes and status in taxonomy l 1．1．2 Classification and Indentification ofEPNS ．．3 1．1．3 Biological characteristics ofEPNs ． ． ．4 1．1．4 Ecological characteristics ofEPNs ．．5 1．2 Application of EPNs in Biological pest control ．．6 1．3 Prospect ofApplication ． ．7 1．4 Purpose and significance ofthis study ． 8 2 Materials and methods ． ． ．9 2．1 Materials 9 2．2 Mehods ．．9 2．2．1 Resources investigation of EPNS ．．9 2．2．2 Screening offine strains ofEPNS ．．11 2．2．3 Molecular identification of EPNs ．．11 2．2．4 Biological characteristics ofEPNs ．12 3 Results analysis ． ．15 3．1 Resources investigation ofEPNs ．15 3．1．1 Distribution ofEPNs 11； 3．1．2 Relationships between EPNs and vegetations ．．15 3．1．3 Relationships between EPNs and soil type ．16 3．1．4 Relationships between EPNs and moisture 16 3．2 Screening offine strains ofEPNs 17 3．3 Molecular identification ofEPNS 18 3．4 Biological characteristics ofnematode JNR ． ．：!：1 3．4．1 Infectivity of nematode JNR ．22 3．4．2 Heat tolerance ofnematode JNR ．23 3．4．3 Infectivity ofnematodeⅢR under high temperature 24 3．4．4 Heat tolerance ofhematode JNR under 10W temperature ， ． ．．25 3．4．5 Infectivity ofnematodeⅢR under low temperature ．25 3．4．6 Infectivity ofnematode JNR under difierent moisture ．26 3．4．7 Movement ability of JNR ．．：16 3．4．8 Infective cycle of JNR ．27 3．4．9 Virulence of JNR grubs ．28 III 万方数据 东北农业大学农学硕士学位论文4 东北农业大学农学硕士学位论文 4 Discussion ．．：19 4．1 Re$ources investigation ofEPNs ．29 4．2 Screening of fine strains ofEPNs ：19 4．3 Biological characteristics of nematode JNR ．．3() 5 Conclusion ．32 Acknowledgement．． ．．．．．．．． ．．．． ． ．．． ．．． ．．．．．． ．．． ．．．．．． ．．．．33 References ．． ．． ． ．．．． ．．．． ． ．． ．．．．． ．． ．．．．．．． ． ． ． ．34 Appendix ．．． ．．． ．．．．． ． ．． ．． ． ．．．．． ．．．．．．． ．．．． -．． ． ．42 Papers published in the pe