山羊瘤胃和草鱼肠道微生物分子生态研究及β葡萄糖苷酶基因的克隆-生物化学与分子生物学专业论文.docxVIP

AbstractThe Abstract The gas臼ointestinal(GO microbinta of animals is characterized by its high population density,wide diversity and complexity of interactions．Importantly，GI microbes have a profound influence on nutritional，physiological and immunological processes in the host．Recently，findings from culture-based methods have been supplemented with molecular ecology techniques that are based on the 16S rRNA gene．These techniques enable characterization and quantification ofthe microbiota，and also provide a classification scheme to predict phylogenetic relationships．Microbial community structure call be analyzed via fingerprinting techniques．Microbes inhabiting the gastrointestine of phytophagous animals conduct a multitude of biochemical reactions，including degrading of cellulose and hemicellulose．The beneficial effects of number of fibrolytic enzymes produced by microorganisms have been investigated and confirmed in several areas of animal production．Thus，study oil the diversity of microorganisms and fibrolytic enzymes in gastrointestine of phytophagous animals as well as analysis of gene function present the indispensably important significance． This thesis aimed at studying microbial community in animal gastrointestinal samples．Separation of polymerase chain reaction(PCR卜amplified 16S rDNA products using denaturing gradient gel electrophoresis(DGGE)was tested means．DNA extraction from the lumen of three species of goats(Capra hircus)(Boer goat，Nanjiang yellow goat，Inner Mongolia cashmere goat)was followed by PCR amplification of 16S rDNA gene，beta subunit of the RNA polymerase(rpoB)gene，and ciliate 18S rDNA gene．PCR products were analyzed by DGGE to compare the predominant bacterial and ciliate community structure．The bacteria community structures of different goats were similar to each other．The similarity of intraspecies was noticeably higher than that of interspecies．Goat species was Ibund to be the factor influencing the runlen microbial community．In contra