利用玉米秸秆发酵生产燃料乙醇的研究-发酵工程专业论文.docxVIP

【II东轻工业学院硕士学位论文摘要 【II东轻工业学院硕士学位论文 摘要 本文对玉米秸秆发酵生产燃料乙醇进行了系统的研究。通过实验确 定了玉米秸秆稀硫酸预处理最适条件、纤维素酶解预处理玉米秸秆的最 适条件；从自然界里筛选出一株发酵木糖产乙醇酵母并对其进行了形态 观察和生理生化实验，初步确定该菌株为奥默毕赤氏酵母；最后利用正 交试验对玉米秸秆发酵生产燃料乙醇的发酵条件进行了研究并建立了 发酵过程的数学模型。 本实验的主要结论： 1．玉米秸秆稀硫酸预处理最适条件：硫酸浓度l-O％，水解温度Ⅲ) ℃，水解时间120min，秸秆粉粒度介于20～40目之间，固形物含量10％。 水解液中的还原糖主要为木糖与葡萄糖。 2．预处理玉米秸秆纤维素酶解的最适条件：纤维素酶用量25IU／g， 水解温度为50。C，水解时间为24h，pH值为4．6。 3．确定所筛选菌株为奥默毕赤氏酵母(Pichia ohmeri)。该菌种发酵 8％葡萄糖产乙醇量为理论产率的76．1％，发酵8％木糖产乙醇为理论产 率的63．6％。 4．利用奥默毕赤氏酵母发酵稀硫酸预处理玉米秸秆水解液生产燃料 乙醇的最佳发酵条件为：发酵温度36℃，发酵时间60h，通风量 O．08m3“m3·min)，初始还原糖浓度为74．ogm。 5．通过正交试验法确定了利用酿酒酵母发酵预处理玉米秸秆滤渣的 最适发酵条件：发酵温度为34℃，发酵时间60h，接种量4×106cell／mL， 纤维素酶用量401U／g。并对该发酵过程进行了理论探讨，建立了发酵过 程的数学模型并通过最小二乘法确立了同时糖化发酵过程中，乙醇浓度、 玉米秸秆固形物含量与发酵时间之间关系的拟合方程。拟合方程为： r=一178．843in0～3．63y)一2．358yc60 关键词：玉米秸秆燃料乙醇奥默毕赤氏酵母正交试验 山东轻工业学院硕士学位论文Abstract 山东轻工业学院硕士学位论文 Abstract The fuel ethanol production with corn stalk was discussed in the thesis． The optimal pretreatment conditions of the corn stalk by the dilute sulfuric acid and the 0ptimal hydr0137sis conditions of the pretreated stalk by the cellulase were determined in the thesis．A strain that COUld ferment the xylose to eth83101 was screened and it was identified as崩chia ohmeri．At the last part of the thesis．the fermentation conditions of the fuel ethanol production by the corn stalk were studied by the orthogonal experiment and mathematic model was established． The major conclusions ofthe thesis included the following five pans： Firstly,the following was廿le optimal conditions of the pretreatment： C(}12S04)=1．0％，the hydrolysis temperature 120。C，the hydrolysis time 2h， the granularity 20～40 meshes and the solid content no more than lO．O％．The major inducing sugars in the hydrolysis liquid were xylose and glucose． Secondly,the optimal hydrolysis conditions of the pretreated corn stalk with cellulase were made：the cellulase weight is 25IU／g com stalk，the temperature iS 50℃．the hydrolysis time iS 24h and the pH valHe iS 4．6． Thirdly,the screened strain was identified Pichia ohmeri and t