扫描电化学显微镜与表面等离子体共振光谱联用技术的开发与应用-分析化学专业论文.docxVIP

硕士学位论文 硕士学位论文 摘要 EC．SPR响应。将双层膜用于细胞色素c氧化还原体系研究，发现其 最大SPR响应是金膜的2．7倍。双层膜的应用对于将SPR技术用于 电极反应灵敏检测的研究具有重大意义。 关键词：扫描电化学显微镜，表面等离子体激元共振，联用技术，双 层金银膜 lI 硕士学位论文 硕士学位论文 ABSTRACT ABSTRACT Surface plasmon resonance(SPR)is all optical technique that can monitor interfacial reactions at solid．1iquid interfacese．SPR iS attractive owing to several inherent advantages，such as label—free analysis，rapid and high sensitivity．In rencent years，SPR has successfully applied to electrochemistry field．EC．SPR offers a viable avgnue to probe optical and electrochemical properties of adsorbares and a sensitive means to quantify thickness variations of ultrathin films accompanying redox reacti0115．However,in conventional EC—SPR experiments，the SPR angular sllifts caused by all externally applied electrode potential could affect the sensitivity．It becomes dimcult for EC—SPR to observe SPR angular shifts caused by conformational change of organic or even biological adsorbates． Two approaches to mitigate the inherent problem of EC·SPR by enhancing the sensitivity are described证t11is thesis．One iS to establish a novel combined technique to avoid the effect of the applied electrode potential on the variation of the surface charge density and reduce the SPR angular shifts．Another is to fabricate novel SPR substractes to enhance the small SPR responses． 111e combination of scanning electrochemical microscopy with surface plasmon resonance iS descibed in the thesis．An oxidant (reduetant)eleetrogenerated at the SECM tip rapidly diffuses to the SPR substrate and oxidizes(reduces)the molecules a伍xed onto the surface． 砀e coupled technique eliminates the SPR resonance angle change caused by all externally applied electrode potential．rnle results．using the ferrocenyl alkanethiol and Ce(N03)3 as a model system，by oxidi