蜡样芽孢杆菌m22超氧化物歧化酶（sod）基因在毕赤酵母中的表达-植物病理学专业论文.docxVIP

AbstractSuperoxide Abstract Superoxide dismutase(EC I．I 5，I，l；SODl is a metal·containing enzyme that widely exist in organisms．It catalyzes the dismutation of superoxide free radical and produces hydrogen peroxide and oxygen．It plays∞important role in protecting the plasmalemma from the reactive oxygen species． A recombinant plasmid pPICZA·sodM l 8 was constructed and after its linearization was transferred into chromosome of Pichia pastoris GS ll 5 by electroporation．The positive transformants with the integrates Mn-sod gene was identified by Zeocin·resistance，PeR screening and expression in