类芽孢杆菌BD3526发酵上清对植物乳杆菌ST-Ⅲ的促生长研究.PDF

1 2016, Vol.39 No.6 乳业科学与技术 ※基础研究 类芽孢杆菌BD3526发酵上清对植物乳杆菌 ST-Ⅲ的促生长研究 * 洪 青，杭 锋 ，吴正钧 （光明乳业股份有限公司乳业研究院，上海乳业生物工程技术研究中心，乳业生物技术国家重点实验室，上海 200436 ） 摘  要：研究类芽孢杆菌BD 3526脱脂乳发酵上清对植物乳杆菌ST-Ⅲ在脱脂乳中的生长影响。结果表明：培 养5 ～9 d 的BD3526 脱脂乳发酵上清液可显著促进植物乳杆菌ST-Ⅲ在脱脂乳中的生长和凝乳。与空白对照相 比，添加含5 ～9 d 发酵上清的脱脂乳培养24 h 后，pH 值从6.44 降至4.70 ～5.08 ，16 h发生凝乳，活菌数达到 9.22～9.35 （lg （CFU/mL ））。 关键词：发酵上清；蛋白水解；植物乳杆菌ST-Ⅲ Growth Promotion of Lactobacillus plantarum ST-Ⅲ by the Fermented Supernatant of Paenibacillus spp. BD3526 * HONG Qing, HANG Feng , WU Zhengjun (State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy and Food Co. Ltd., Shanghai 200436, China) Abstract: The growth promoting effect of the supernatant of fermented skim milk with Paenibacillus spp. BD3526 on Lactobacillus plantarum ST-Ⅲ inoculated into skim milk was analyzed in this study. The results showed that the supernatant of BD3526 cultured in skim milk for 5–9 d significantly promoted the growth of ST-Ⅲ in skim milk and exhibited high milk- clotting activity. After ST-Ⅲ was cultured in skim milk added with the supernatant of BD3526 for 24 h, the pH value decreased from 6.44 to 4.70–5.08, and milk clotting was observed at 16 h along with a viable cell count of 9.22–9.35(lg(CFU/mL)). Key words: fermented supernatant; proteolysis; Lactobacillus plantarum ST-Ⅲ DOI:10.15922/ki.jdst.2016.06.001 中图分类号：TS252.1 文献标志码：A 文章编号：1671-5187 （2016 ）06-0001-05 引文格式： 洪青, 杭锋, 吴正钧. 类芽孢杆菌BD3526发酵上清对植物乳杆菌ST-Ⅲ的促