山萘酚对缺血再灌注脑损伤大鼠的保护作用-神经病学专业论文.docxVIP

浙江大学医学院硕士学位论文结果： 浙江大学医学院硕士学位论文 结果： 缺血再灌注损伤大鼠的脑组织中MDA含量明显增高，而NO含量， SOD、GSH-px活性显著降低，免疫组化技术及TUNNEL技术观察可见半胱氨 酸蛋白酶．3阳性细胞增加，细胞凋亡明显增加。山萘酚能明显减少缺血再灌注 损伤大鼠的脑组织中MDA含量，并增加NO含量和SOD、GSH-px活性，半胱 氨酸蛋白酶．3阳性细胞减少，凋亡细胞也明显减少。 结论： 山萘酚对缺血再灌注损伤大鼠有保护作用，其作用可能与抗氧化，抑 制半胱氨酸蛋白酶．3，从而减少脑细胞凋亡有关。 关键词：山萘酚；缺血再灌注；氧自由基；半胱氨酸蛋白酶．3；细胞凋亡 浙江大学医学院硕士学位论文The 浙江大学医学院硕士学位论文 The protective effect of kamperol on focal cerebral ischemia i11 rats Department ofneurology, Medical College of Zhejiang University Postgraduate He Yuzhou Supervisor Ding Meiping Abstract Background Irreversible brain disorder usually comes occur due to the ischemical reperfusion injury．A large amount of basic and clinical researches had been made for,but there has no breakthrough progress comes still．The study of molecular biology demonstrated that multiple factors involved in the process of cerebral ischemia reperfusion injury,apoptosis，free radical damage，calcium overload，excitotoxicity of amino acids and others have been validated by most experiments．The death of nerve cell after brain irljury can be divided into two types：cellular necrosis and apoptosis，which play a key role in secondary injury．The primary injury is unavoidable．so to block nerve cell apoptosis in the secondary injury in early stage become the important turning point in the protection of 浙江大学医学院硕士学位论文brain 浙江大学医学院硕士学位论文 brain injury． Aim：To investigate whether kaempferol exerts a neuroprotective effect on focal cerebral iSChemia in normotensive rats． Methods：The model of focal cerebral ischeIllia in rats was established by middle cerebral artery occlusion(MCAO)for 90 min followed by perfusion．Rats are randomly divided into sham-operated group，focal cerebral ischemia group，kaempferol·treating(10mg／kg)groups，and positive control(Edaravone-treating)group．Rats were killed and brain samples were collected for the measurement of infarct size and the content of super oxide dismutase，glutathione peroxidase(GSH-Px)，nitric oxide，malondialdehyde(MDA)．Furthermore，the expression o