量子点荧光微球用于猪繁殖与呼吸综合征检测新方法研究.pdfVIP

量子点荧光微球用于猪繁殖与呼吸综合征检测新方法研究 3 实验方法 ··················································································· 41 3.1 荧光微球探针的合成 ···························································· 41 3.2 免疫检测的步骤 ·································································· 41 4 结果与分析 ················································································ 42 4.1 pH 的优化 ·········································································· 42 4.2 Rhod-5N 对于 Cd2+ 的特异性研究 ············································· 43 4.3 Rhod-5N 对于 Cd2+ 的检测 ······················································ 44 4.4 PRRSV 血清中抗体的检测 ····················································· 45 5 结论 ························································································· 46 全文总结 ···························································································· 47 参考文献 ···························································································· 48 致谢 ·································································································· 58 iii 万方数据 量子点荧光微球用于猪繁殖与呼吸综合征检测新方法研究 摘要 猪繁殖与呼吸综合征( Porcine reproductive and respiratory syndrome, PRRS)又称 蓝耳病，是由猪繁殖与呼吸综合征病毒(PRRSV) 引起的一种急性传染病。该病流行 范围广，死亡率高，给我国养猪业带来了巨大的经济损失，成为我国养猪业中危害 最大的传染病之一。因此发展和建立一种快速、高灵敏的检测方法对于猪繁殖与呼 吸综合征的诊断与控制具有重要的意义。 随着纳米科技的不断发展，基于信号放大的纳米材料的合成以及分析应用成为 研究热点。量子点荧光微球作为一种荧光以及电化学信号放大的复合材料在生物成 像、疾病检测等方面取得了一些进展，但在动物重大疾病的检测方面研究的较少。 本文合成了量子点包裹的二氧化硅微球，并偶联蛋白，采用间接荧光免疫分析法 （Fluorescence-linked immunosorb