联合共培养系统诱导骨髓间充质干细胞分化为视网膜色素上皮细胞研究-老年医学(眼科学)专业论文.docxVIP

6 6 co-culture with human retinal photoreceptor cells. PEDFs ELISA detection is positive in Extracting supernatant of RPE-like cells. Conclusion HRPE+BDNF、EGF、bFGF + BMSCs can induce BMSCs differentiate into RPE-like cells．HRPE and systematic stir-in concentration in BFGF, BDNF factor, EGF can induce people BMSCs differentiate into RPE similar cells and express surface protein (keratin18 and RPE65);to engulf shedding outside membrane of retinal receptors and secrete PEDF function. Key words bone marrow stromal cells ； human retinal pigment epithe — lialium；co-culture；Induced differentiation