脂代谢紊乱对大鼠肾小管上皮细胞roat3基因表达新.pdfVIP

脂代谢紊乱对大鼠肾小管上皮细胞rOAT3基因表达的影响 摘要 系)为对照。基础饲辎饲养至7周龄，剪尾取血保存，1l周龄时所有大鼠用水 合氯醛腹腔注射麻醉，左肾静脉取血，活体取肾皮质液氮保存，采用生化仪检测 Jf【L脂、尿酸、血糖、尿素氮、肌酐，采用逆转录一聚合酶链反应(RT—PCR)方法 组间两两比较选用t检验。 ：PO．05)；血糖、尿素氮、肌酐、血尿酸水平与对照组LETO大鼠相比无明显差 耳。 小管上皮细胞rOAT3基因表达减弱与尿酸排泄减少有关，脂代谢紊乱与痛风及高 尿酸血症的发病相关。 关键词：脂代谢紊乱：OLETF大鼠；rOAT3；基因表达 on Theinfluenceof rOAT3inrenaltubular of dislipidemia epithelia rats ABSTRACT theinfluenceof on Objective：Toinvestigate hyperlipidemiarOAT3(rat renaltubular ofrats．Tomakeclearthe organicaniontransporter3)inepithelia between andthe of and dislipidemiadevelopmentgout relationship hyperuricemiaby animal experiments． includedtenfour—weeks-oldmaleOLETFrats， Methods：Experimentalgroup control includedtenfour-week—oldmaleLETOratswhich tothe group belonged samekindwithOI，ETF wereall treatmentforseven rats．They givenadaptive weretakenoutbloodfrom thetime thetails week．Reserve weeks，duringthey every serum fromtheblood．Treateleven-week·oldratswithchloral the seperated hydrate fromtheleft inabdominal blood vein，andtakeout byrejection cavity，take kidney some cortex．．Theconcentrationofserum acidand were kidney lipid、uric glucose determined met