miRNa靶标预测及验证-公开课件.pptVIP

miRNA 靶标预测和功能验证 miRNA 的靶标预测 Seeds of miRNA Target Prediction UTR Context Target-Prediction Tools 大量的miRNA 被发现，产生了许多的问题，这个非编码的小RNA 在细胞中的作用是什么？ 回答这个问题的关键是要找到miRNA 所调节的基因。在植物中靶标基因与miRNA是互补很容易预测到，但是在动物中只是偶尔才发现。 现在预测的方法有很多但是有三个结论是实验验证过的。 The first major conclusion is that requiring conserved Watson–Crick pairing to the 5′ region of the miRNA centered on nucleotides 2–7, which is called the miRNA “seed” The second conclusion is that conserved pairing to the seed region can also be sufficient on its own for predicting conserved targets above the noise of false-positive predictions The