罗氏荧光定量PCR.ppt

ET: PCR Efficiency for traget molecules ER: PCR Efficiency for reference molecules Cp: Amplification cycle at crossing point; CT: Threshold Cycle S: Sample C: Calibrator The error generated when assuming the efficiency of a PCR reaction is = 2 but the actual efficiency is = 1.95. After 35 cycles of PCR, the quantified amount of sample material will have 142% error, a greater than 2-fold difference than the actual amount. Further down in this table, if you look at a PCR efficiency of 1.8, the error at 35 cycles is nearly 4000%. * RT时做2-3个平行反应，每个样本采用相同的RNA浓度 When a mu