线阵CCD光纤荧光传感系统研制与应用.pdfVIP

摘要 实验研究了叶绿素的荧光光谱的测定、单一浮游植物荧光的测定、浮游植 物溶液的测定及标定。通过实验表明，该光纤荧光生物传感系统利用浮游植物 的荧光激发光谱，对其进行分类识别，检测快速，可用于现场在线测定。今后 还要改进、完善系统的硬件和软件，提高浮游植物分类识别的数量和准确率， 实现实验室样机的工程化和商品化，为水质监测等海洋应用提供新的技术手段。 关键词：浮游植物 荧光检测 LED光源 CCD 全光纤光路 Ⅱ Abstract Abstract As the main primary producers in the oceans, marine phytoplankton provides about 90 to 95 percent of marine primary production. Monitoring types and quantities of phytoplankton is a routine basic work in marine scientific research. The traditional methods of monitoring are microscope count method and standard precipitation count method, which needs consuming time and requires the staff with high professional quality. In addition, the measurement results using the traditional methods can not represent the concentration of large algae waters ,depict its distribution, and meet the statistical analysis of a large number of samples because of the large randomness.Due to high sensitivity properties and multi-channel characteristics, low noise detection and signal synchronization determination, CCD can achieve full spectrum measured. So in the project system fiber optic fluorescence biosensor uses CCD detector technology. CCD have low illumination device and good linear ratio, which can improve spectrum efficiency and anti-jamming capability of fiber -optic system and reduce the cost of the system. With the unique advantages fluorescent fiber sensor can be applied in many ways, especially in extreme environments and conditions. Fluorescence monitoring seaweed state mainly measure fluorescence excitation spectrum and fluorescence spectrum of seaweed under the