Bioseparations Engineering生物分离工程.pptVIP

Bioseparations Engineering ;Why? ;= $ 1.32 million per carats ≈ $6.6 million per gram;Heparinase I (肝素酶 I) = $ 450 /100 ng = $4500, 000, 000/g =$ 4500 million per gram;It is difficult to separate them:;Chapter 1 Introduction;What is Bioseparations?; Extracted from whole plants and animals (提取) synthesized in bioreactors containing cells or enzymes (生物合成);What is Bioseparations;What is Bioseparations;Equipment Hardware, bioreactors, software and consumables supporting biotechnology; Biotechnology has three-component core: ;Above all: ;Upstream Processing;What is Bioseparation;The characteristics of Bioseparation;;Small Biomolecules;Small Biomolecules;Small molecules ;Date;Proteins;The characteristics of Bioseparation;Nucleic acids and Oligonucleotides;The characteristics of Bioseparation;Polysaccharides;★ low concentration of the targets (目标产物低浓度);Table typical products from fermentation;★ Fermentation broth being a complicated mixture (complex components) (发酵液成分复杂);★ Poor stability (稳定性差); The diversity of products spawns (results in) the broad spectrum of separation methods used;;★ These separations are elaborate and expensive;☆ Yeast biomass, penicillin G, and certain enzyme processing costs are percentages of the selling prices which are 20%, 20-30% and 60-70% respectively.;Price vs Production; The recovery and the purification processes must be well conceived and well designed!;So, Before designing, what we should ask and answer are:; What are the unusual physicochemical properties of the product and the principal impurities? (产物及主要杂质独特的物化性质是什么？) What are the economics of various alternative separations? (各种分离方法的经济性比较);Improve the efficiency of bioseperation Reduce the steps of operations Reduce the time of operation process; Remove the insolubles (不溶物的去除） Filtration [过滤] Centrifugation [离心] Cell disruption (nece