文件号10389-3-e-1pdf福建农林大学生物学实验教学资料精.pdf

文件号：10389-3-e-1.pdf 福建农林大学生物学实验教学中心 生物学实验讲义 目 录 第一篇 植物学部分 实验1 光学显微镜技术··················································· 1 实验2 显微摄影技术····················································· 4 实验3 徒手切片法······················································· 6 实验4 石蜡制片法······················································· 7 实验5 临时装片技术及植物细胞和生命现象观察 ························· 10 实验6 植物组织的结构与观察··········································· 15 实验7 根的形态和结构的观察··········································· 18 实验8 茎的形态和结构的观察··········································· 23 实验9 叶的形态和结构的观察··········································· 27 实验10 营养器官的变态类型的观察······································ 30 实验11 被子植物的生殖器官············································· 33 实验12 种子和幼苗······················································ 38 实验13 低等植物的分类················································· 42 实验14 高等植物的分类················································· 48 实验15 被子植物分类的形态学依据 ····································· 52 实验16 分类检索表的应用以及植物种的描述记载方法····················61 实验17 离瓣花亚纲的分类鉴定··········································· 63 实验18 合瓣花亚纲的分类鉴定··········································· 64 实验19 单子叶植物纲的分类鉴定········································ 64 实验20 植物的识别·················································65 附录一 种子植物主要科代表植物名录 ···································· 65 实验21 植物腊叶标本的制作及鉴定 ····································· 78 第二篇 生物化学部分 实验21 不连续 SDS-聚丙烯酰胺凝胶电泳································· 82 实验22 酵母 RNA的提取与分离·········································· 83 实验23 大蒜细胞 SOD 的提取和分离····································· 84 实验24 多糖的水解及水解物的薄层层析测定····························86 实验25 菜花（花椰菜）中核酸的分离和鉴定····························88 第三篇 分子生物学部分 实验26 质粒DNA 的提取················································ 90 实验27 琼脂糖凝胶电泳检测 DNA········································ 91 实验28 PCR基因扩增····················