毕业设计论文-中国试验方剂学杂志.doc

PAGE 9 中药胃肠舒对糖尿病大鼠胃窦平滑肌细胞线粒体的影响[基金项目] [基金项目]国家自然科学基金资助项目( NONO，烟台市科技攻关项目( NO. 2008162)，大学生科技创新计划（BY2007DKCX10） [作者简介] 宋晓冬（1969-），女（汉族），硕士生导师，副教授，从事方向：药物机理 Corresponding. Tel: 0535-6913074,E-mail: songxd71@ [通讯地址]山东省烟台市莱山区观海路346号，邮编264003 宋晓冬 张瑾锦 刘文波 王秀文 滨州医学院实验中心，山东，烟台，264003 [摘要] 目的：研究中药胃肠舒对糖尿病（diabetes mellitus, DM）大鼠胃窦平滑肌细胞线粒体形态、结构、功能和蛋白的影响，探讨胃肠舒促胃肠运动的线粒体动能调控机制。方法：选择体重150-170g的标准SD大鼠随机分为4组：正常对照组、DM组、DM+胃肠舒Ⅰ组、DM+胃肠舒Ⅱ组。透射电子显微镜检测线粒体形态、结构的变化，流式细胞术检测线粒体跨膜电位的变化，荧光测定法检测线粒体呼吸链的变化，Western Blot检测线粒体蛋白的变化。结果：胃肠舒能改善DM大鼠胃窦平滑肌细胞线粒体的形态、结构，增强呼吸链功能，线粒体跨膜电位和ATP含量增高，细胞色素C氧化酶(cytochrome C oxidase, COX)活性增强，ROS释放减少，凋亡蛋白家族Bcl-2表达上调，Bax表达下调。结论：胃肠舒促胃肠运动可能与改善平滑肌 关键词：中药胃肠舒；胃肠运动；线粒体；跨膜电位；呼吸链 Effect of Chinese Medicine Weichangshu on Mitochondrial in Diabetic Mellitus Rats Stomach Smooth Mucle Cells SONG Xiao-dong ZHANG Jin-jin LIU Wen-bo WANG Xiu-wen Experiment Center of Binzhou Medical College, 【Abstract】Objective To study the mechanism of the chinese medicine Weichangshu on mitochondrial kinetic energy in diabetic mellitus rats stomach by measuring the changes of morphology, structure, function and protein of mitochondria. Methods: Standard Sprague Dawley rats with a mean weight of 150-170g were randomly divided into 4 groups, including a control group, DM model group, DM+ WeichangshuⅠgroup, DM+ Weichangshu Ⅱ group. The mitochondrial morphology and structure were detected using transmission electron microscopy. The changes of mitochondrial transmembrane potential, respiratory chain and mitochondrial protein were detected using flow cytometry, fluorescence labeling and Western Blot respectively. Results: Weichangshu could ameliorate the mitochondrial morphology, structure and respiratory chain in diabetic mellitus rats stomach. Mitochondrial transmembrane potential, ATP level and activity of cytochrome C oxidase were increased. The release of ROS was decreased. The expression of Bcl-2 up-regulated and bax down-regulated. Conclusion: Weichangsh