曼陀罗种子萌发及植株再生.pdf

中草药 Chinese Traditional and Herbal Drugs 第 43 卷 第 12 期 2012 年 12 月 • 2499 • 曼陀罗种子萌发及植株再生 张 鸿，张显强，罗正伟，谌金吾，孙 敏* 西南大学生命科学学院 三峡库区生态环境教育部重点实验室，重庆 400715 摘 要：目的 探索曼陀罗种子萌发及组织培养条件的优化。方法 利用不同质量浓度 GA3 及不同处理时间的曼陀罗种子， 测定其发芽率和发芽势；采用正交设计法探讨不同植物激素配比及培养基对曼陀罗再生体系建立的影响。结果 种子用 60 mg/L GA3 浸泡 10 h 后发芽率最高，达到 73.33% ；叶片用75%乙醇消毒 15 s 后用 0.1% HgCl2 处理 3 min 时成活率最高，为 70.59% ；以叶片为外植体诱导出愈伤组织的最佳培养基为MS ＋0.5 mg/L 6-BA ＋0.1 mg/L NAA，诱导率达 92.5% ；丛生芽诱 导的适宜培养基为 MS ＋2.0 mg/L 6-BA ＋0.1 mg/L NAA，诱导率达到 94.50% ；生根为不加激素的MS 培养基，15 d 后根系 粗壮，移栽成活率达 90% 以上。结论 使用 GA3 促使曼陀罗种子快速萌发，用“两步法”建立了曼陀罗高效无菌再生体系。 关键词：曼陀罗；种子萌发；组织培养；植株再生；最佳培养基 中图分类号：R282.21 文献标志码：A 文章编号：0253 - 2670(2012)12 - 2499 - 04 Seed germination and plant regeneration of Datura stramonium ZHANG Hong, ZHANG Xian-qiang, LUO Zheng-wei, CHEN Jin-wu, SUN Min Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Southwest University, Chongqing 400715, China Abstract: Objective To optimize the conditions for the seed germination and plant regeneration of Datura stramonium. Methods The seeds were treated by GA3 at different concentration for various durations, the germination percentage and germination trend of seeds were determined, and the effects of different hormones and media on the plant regeneration were investigated by orthogonal design. Results The seeds treated by GA3 (60 mg/L) for 10 h had the highest germination percentage (73.33%); The leaves had the highest survival rate (70.59%) when sterilized in 75% ethyl alcohol for 15 s then 0.1% HgCl for 3 min; The optimal medium for callus induction of leaves was MS + 0.5 2 mg/L 6-BA + 0.1 mg/L NAA, with the inductivity of 92.5%; The optimal medium for callus induction of clustered buds was MS + 2.0 mg/L 6-BA + 0.1 mg/L NAA, with the inductivity of 94.50%; The rooting was radicated in h