有机磷农药酶生物传感器研究进展.pdf

第 18 卷 第 4 期 化　学　进　展 Vol. 18 No. 4 2006 年 4 月 PROGRESS IN CHEMISTRY 　Apr. , 2006 有机磷农药酶生物传感器研究进展 王丽红 　张　林　陈欢林 (浙江大学材料与化工学院生物工程研究所 　杭州 310027) ( ) ( ) 摘　要 　酶生物传感器 EBS 以简单、廉价、易于微型化等优势成了有机磷农药 OPs 传统分析方法的 最佳替代品。本文从识别 OPs 的酶及识别机理、电化学 EBS 、酶的固定化技术、高分子材料的酶固定载体不 同角度综述了有机磷农药酶生物传感器研究近况 ,并重点介绍了一次性丝网印刷酶电极。 关键词 　酶生物传感器 　有机磷农药 　酶固定化技术 　酶固定载体 　丝网印刷酶电极 ( ) 中图分类号: O62751 ; TQ45 ; TP2123 　文献标识码 : A 　文章编号 : 1005281X 2006 Enzymatic Biosensors for Detection of Organophosphorus Pesticides Wang Lihong 　Zhang Lin 　Chen Huanlin (Department of Biological Engineering , College of Materials Science Chemical Engineering , Zhejiang University , Hangzhou 310027 , China) Abstract 　Enzymatic biosensor ( EBS) is the most promising substitute for traditional analytical methods on ( ) determination of organophosphorus pesticides OPs . The merits of simple , inexpensive and miniature make it potential for environmental monitoring. Enzymatic determination of OPs is often based on cholinesterase (acetylcholineaterase and butyrylcholinesterase) and organophosphorus hydrolase. Various methods have been applied for enzyme immobilization in the construction of EBS for determination of OPs. Since the chemical and physical properties of the polymers may be