荧光光谱在蛋白质研究中的应用.pptVIP

Application of FRET to the GroEL–GroES Chaperonin Reaction, GroEL (EL315C + IAEDANS) EL513-D, GroES(ES98C + F5M) ES98-A METHODS 24, 278–288 (2001) 6、绿色荧光蛋白 绿色荧光蛋白空间结构 (Green Fluorescence Protein): 1962年在水母中分离得到。它由11个?-折叠围绕成一个?-折叠桶，结构中唯一的一个?-螺旋在桶的中间。 The mature protein resists many proteases and is stable up to 65?C and pH 5 to 11, in 1% sodium dodecyl sulfate or 6M guanidiniam chloride. Fluorescent is optimal at pH 7.2 to 8.0. It requires only UV or blue light and oxygen for fluorescence without any cofactors or substrates. ?-螺旋包括一个氨基酸三联体：residues 65-67, Thr-Tyr-Gly