酪氨酸-钐Ⅲ配合物与鲱鱼精DNA的作用方式-中南大学学报.pdf

41 3 () Vol.41 No.3 2010 6 Journal of Central South University (Science and Technology) Jun. 2010 -( )DNA 1 1 1 1 2 (1. 621010 2. 610041) UV pH=7.40 Sm()Tyr() n(Sm())׃n(Tyr)=1׃3Sm()(Tyr) hs()DNA n(Sm()(Tyr) )׃ 3 3 Θ 4 Θ 3 n(DNA)=3׃1K 298.15 K =9.9710 L/mol K 310.15 K =7.5610 L/mol Sm()(Tyr)3 hsDNA Scatchard Sm()(Tyr)3 hsDNA () DNA O629.74 A 1672−7207(2010)03−0876−07 Binding mode between Tyr-Sm() complex and herring sperm DNA 1 1 1 1 2 ZHAO Na , WANG Xing-ming , ZHANG Yan , HU Ya-min , DING Li-sheng (1. School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China; 2. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China) Abstract: With the methods of UV spectra, fluorescence spectra in physiological pH environment (pH=7.40), the binding ratio of n(Sm())׃n(Tyr)=1׃3 and n(Sm()(Tyr)3)׃n(DNA)=3׃1 were confirmed by molar ratio method. The binding Θ 4 Θ 3 constants of K 298.15 K =9.9710 L/mol and K 310.15 K =7.5610 L/mol were confirmed by double reciprocal method. Thermodynamic function computation demonstrates that ∆ H Θ is the primary driving power of the interaction of r m Sm()(Tyr)3 and DNA. Combined with Scatchard method and viscosity method, the results s