微生物学美国IndianaUniversityPurdueUniversity授课04.pptVIP

Strategies for StudyingMicrobial Pathogenesis BIOL 533 Lecture 4 Reporter Gene Fusions Operon fusion: transcriptional Promoter from foreign gene Translational sequence from reporter gene Reporter Gene Fusions Gene fusion: translational Promoter and translational signal from foreign gene Initiation signal/+NH2 coding sequence Translational signal also from reporter gene Initiation sequence and part of NH2 sequence of reporter gene missing, but COOH region still present Two coding sequences must be in frame Technique Use transposon vector containing lac fusion Carries promoterless lacZ gene