细菌脂多糖免疫对结肠炎小鼠TNFα表达的影响2汇总.docVIP

细菌脂多糖免疫对结肠炎小鼠TNF-α表达的影响 [摘要]目的：为了研究细菌脂多糖免疫对结肠炎小鼠TNF-α表达的影响并探讨炎症性肠炎的发生机制。方法：设计动物模型实验，将30只BALB/C小鼠随机分为三组：观察组10只（注射细菌脂多糖免疫后诱导小鼠溃疡性结肠炎模型）、模型组10只（注射生理盐水后诱导小鼠溃疡性结肠炎模型）和对照组10只（不做任何处理并注射生理盐水），4周后处死并取结肠组织标本检测，统计炎症介质TNF-α的表达情况并进行比较。结果：观察组血清TNF-αmRNA含量均值为（16.36±1.28）%，结肠TNF-α含量均值为（23.25±2.15）%，数据结果与其他两组差异具有统计学意义（P0.05）。病理切片观察结果：观察组小鼠的溃疡点、靡烂点个数均较模型组明显减少，同时炎症细胞浸润和杯状细胞减少程度也明显较轻。结论：细菌脂多糖可降低小鼠结肠粘膜TNF-α及血清TNF-α的含量，在病理切片结果中可明显观察到经过LPS免疫小鼠的病理状态明显减轻，这可能为其对溃疡性结肠炎的治疗提供理论依据。 [关键词]细菌脂多糖；溃疡性结肠炎；肿瘤坏死因子 Effect of LSP on the expression of TNF- α immunity in mice with colitis [Abstract] Objective: To study the influence of LSP on the expression of TNF- α immunological colitis in mice and to explore the mechanisms of IBD. Methods: the experiment animal model design, 30 male BALB/C mice were randomly divided into three groups: the observation group 10 (IBD models were induced by injection of LSP after immunization), 10 rats in model group (IBD mice models were induced by injection of saline) and 10 in control group (without any treatment and injection of physiological saline), 4 weeks after the colon tissue samples were taken for detection, and compared the expression of inflammatory mediators in TNF- α statistics. Results: in the observation group, serum TNF- α mRNA content was (16.36 ± 1.28)%, the mean content of TNF- α colon was (23.25 ± 2.15)%, with statistical significance of data and other differences between the two groups (P0.05). Pathological observation: the number of the observation group mice ulcer, corrupt are compared with model group were significantly reduced, and the infiltration of inflammatory cells and goblet cells decreased significantly lighter.Conclusion: LSP can reduce the content of the colon mucosa of TNF- α and serum TNF- α, in pathological results can be clearly observed the pathological state of mice immunized with LPS significantly reduced, which may provide a theoretical basis for the treatment of ulcerative coliti [Keywords]LSP; IBD; tumor necrosis factor 炎症性肠病（inf