组织工程软骨仿生基质材料的构建-第三军医大学学报.doc

利用胶原和聚乙烯醇构建组织工程软骨支架 崔运利，王富友，谭洪波，张 颖，陈卫军，杨 柳 （400038 重庆，第三军医大学西南医院关节外科中心） [摘要] 目的 利用仿生学原理，制备组织工程软骨支架材料。方法 依据人体正常关节软骨细胞外基质主要成分Ⅱ型胶原、硫酸软骨素和透明质酸；添加高分子聚合物聚乙烯醇（PVA）来提高支架强度，应用冷冻干燥等技术制备复合支架，接种细胞体外静态培养7d,通过力学测试、扫描电镜和组织染色等方法对支架进行检测，采用SPSS 13.0 统计软件包进行分析。结果 支架孔隙均匀，孔隙率为93%～95%，CoⅡ-HA-CS-PVA复合支架最大张力约为1.19N，单纯CoⅡ-HA–CS复合支架约为0.55N，差异有统计学意义（t=4.985，P=0.002），细胞在支架上均匀分布，活力旺盛。结论 CoⅡ-HA-CS-PVA复合支架具备良好的生物相容性，可为种子细胞增殖分化提供一个适宜的环境,有很好的临床应用前景。 [关键词] Ⅱ型胶原；硫酸软骨素；透明质酸；聚乙烯醇；组织工程；支架材料 [中图法分类号] [文献标志码] Construction of scaffold for cartilage tissue engineering using collagen and polyvinyl alcohol Cui Yunli，Wang Fuyou，Tan Hongbo，Zhang Ying，Chen Weijun，Yang Liu （Southwest hospital, Third Military Medical University, Chongqing 400038, China） [Abstract] Objective Construct the scaffold for cartilage tissue engineering according to bionics principles. Methods 3-dimensional scaffolds were prepared with type-II collagen, hyaluronan (HA) and chondroitin sulfate (CS), PVA was added to enhance mechanical strength. The scaffolds were manufactured by process of freeze-dry. The scaffolds were evaluated by the SME, mechanical test and immunohistochemistry. Then the 293 cells were seeded in the scaffold and cultured 7 days in vitro. Data was analysed by SPSS13.0. Reasults The pores of scaffold is uniform and the rate of porosity is 93-95%. The maximum tension of CoⅡ-HA-CS-PVA scaffold is 1.19N, and that of the CoⅡ-HA-CS scaffold is 0.55N. the difference was statistically significant (t = 4.985, P = 0.002). Cells are distributed evenly in scaffold. Conclusions The CoⅡ-HA-CS-PVA scaffold can provide a suitable environment for cells proliferation and differentiation. That have very good prospects in the clinical application. [Key words] type II collagen; chondroitin sulfate; hyaluronan; tissue engineering; scaffold material Supported by the National Natural Science Foundation of China ；Supported by the Foundation for Sci Tech Research Project of Chongqing(2009A