褪黑素对小鼠视交叉上核c-fos基因表达的影响.pdfVIP

褪黑素对小鼠视交叉上核c-fos基因表达的影响 Abstract掌 of of effect The molecularmechanism potential anti—aging itscorrelationwithc-fos was melatonin(MT)and generegulation evaluatedinthis c-fos inthe expression study．Thegene and mice mice old after nuclear($CN)of suprachiasmatic young MT under and orsalinetreatmentnormal1ight—darkshifting circumstanceswasobserved． Micewerehousedina roomwith temperature—controlled relatire of50％andwere MTat19：00—20：00 humanity given every to mousebrain make slidesof cryoultramicrotome day．Byusing ofc-Fos wasdone determination SCN，the proteinexpression tothe accordingimmunocytochemical RT—PeR to levelinthe method mRNA SCNwas rapid analysis inour developed1aboratory． Undernormal condition(L：Dwasshown light—dark 12：12)，it that ofc-fos inthe thelevels andmRNA micewere protein young th