铁蛋白的生物工程应用1.pdf

铁蛋白的生物工程应用1 王玲 1,2,3，吴洋 1,2,3，张盛 1,2,3，齐浩 1,2,3** (1天津大学化工学院 天津 300072；2系统生物工程教育部重点实验室 天津 300072；3天津大学化工协同创新中心合成生物学平台 天津 300072) 摘要:铁蛋白是一种普遍存在于生物体内的储铁蛋白，具有铁离子代谢、抗氧化胁迫及消除其他过量金属离子毒害 作用的功能。随着对铁蛋白结构和生化功能认识的深入，铁蛋白作为一个含有四氧化三铁核心的特殊蛋白复合体， 被广泛应用于生物医学、纳米材料、生物分子成像等各种生物工程领域。该综述针对已知的主要铁蛋白分子，论述 了铁蛋白的结构及酶活性机理，基于铁蛋白的多功能分子骨架应用，以及基于铁蛋白磁性的生物分子开关等热点研 究，最后对铁蛋白生物工程、生物医学领域的应用和发展进行了展望。 关键词：铁蛋白 磁性颗粒 分子骨架 中图分类号：Q71 1 BioengineeringApplication ofFerritin v 0 1,2,3 1,2,3 1,2,3 1,2,3** WANGLing ,WUYang ,ZHANG Sheng ,QIHao 6 9 (1.School of Chemical Engineering andTechnology,Tianjin University,Tianjin 300072,China;2.Key Laboratory of SystemsBioengineering ofMinistry ofEducation, 0 TianjinUniversity,Tianjin 300072,China;3.SynBioResearch Platform,Collaborative Innovation Center of Chemical Science andEngineering,Tianjin University,Tianjin 0 . 300072,China.) 4 0 8 Abstract:Widely conserved iron-storage protein plays crucial role in ferric ion metabolism maintaining iron homeostasis, 1 resisting oxidative stress and eliminating other toxic effects of excessive metal ions. With gaining the knowledge from 0 2 structural and molecular studies, iron-storage protein complex in which iron cluster formed from absorbing free iron has : been successfully applied in fields of biomedical engineering, nanomaterial, and biomolecule imaging. Herein, the recent v i progresses in studying the catalytic mechanism of iron cluster formation are briefly introduced. And the cutting-edge X a bioengineering applications in which iron-storage protein were engineered as a versatile molecular scaffold for presenting n special chemicals, or natural magnetic particle for constructing remote control molecular machine are reviewe