黑麦染色体组特异PCR标记地建立与其小麦-非洲黑麦1RS_1BL染色体新易位系地获得.pdf

ABSTRACT transferred in those newly produced lines. 2. The gliadin analysis were carried out on twenty wheat- Secale africanum derivatives L1-L20 by using the Acid polyacrlamide gel electrophoresis (APAGE). Total 24 different electropheratic bands were observed between ωand γ regions, which was indicative of the gliadin gene polymorphism existing the selective lines. In comparison of the standard gliadin patterns, L8, L9, L10, L15and L16 were apparently had the GliB1l, the marker for 1BS/1BL translocation. The result indicated that the above lines are the new type of the S. africanum derived 1RS/1BL lines. 3. Genome in Situ Hybridization (GISH) were carried out on triticale using probe 20H,and the result indicated that probe 20H was dispersed throughout the rye genome except telomeric regions and nucleolar organizing regions. The GISH pattern revealed that these two visible signals appeared on the short arms of line L10. It is obvious that repeat sequence is very important, and need further research. In this study, based on the repeat sequences, new specific PCR primer set was devoeloped, and it was firstly adapted to new materials of L1-L20 for detection of rye introduced to the wheat. Combinging with APAGE and FISH, we concluded that one new wheat-S.cereale 1RS/1BL translocation has been successfully screened. Keywords: Wheat, Rye, Repeat sequences, PCR molecular marker, APAGE, Genome in Situ Hybridization IV 目录 目 录 摘 要I ABSTRACT III 目 录V 第一章 绪论 1 1.1 小麦族植物基因资源 2 1.2 黑麦基因导入小麦材料的研究 3 1.3 外源基因导入小麦的途径 4 1.4 小麦族重复序列的研究 5 1.4.1 小麦及近缘物种基因组中重复序列 5 1.4.2 黑麦基因组中重复序列 6 1.4.3 特异DNA 重复序列作为分子标记检测导入小麦外源染色质 8 1.4.4 DNA 重复序列在小麦遗传学中的应用 9 1.5 小麦背景中外源遗传物质的检测 11 1.5.1 形态学标记 11 1.5.2 细胞学检测 11 常规细胞学标记