放射性粒子125I对胶质瘤细胞治疗作用的试验研究.docVIP

放射性粒子125 I对胶质瘤细胞治疗作用的实验研究 潘宝根1 赵文清1，2 李文玲2 王跃红1 边艳珠3 （1河北医科大学神经外科教研室,石家庄 050031；河北省人民医院：2神经外科，3核放射科，石家庄 050051） 提要：目的 　探讨放射性粒子125I在动物体内抗人脑胶质瘤的效果及其作用机制。　方法　建立人胶质瘤的动物模型，瘤内植入放射性粒子125I，观察肿瘤生长情况，在电镜下观察其超微结构的变化,采用原位缺口末端标记技术检测肿瘤组织的凋亡情况，流式细胞仪检测Bax/Bcl-2的表达，并应用免疫组织化学染色检测血管密度和VEGF、bFGF的表达。结果 人胶质瘤细胞U251的动物模型成功完成, 植入放射性粒子125I后，胶质瘤生长较对照组明显受到抑制，电镜下可呈现核断裂, 核染质边聚等细胞凋亡的形态特征，检测显示肿瘤组织凋亡率以及Bax/Bcl-2明显升高，微血管密度以及VEGF、bFGF表达明显降低。结论　放射性粒子125I对在体人胶质瘤U251有明确的治疗作用，促进瘤细胞凋亡和降低瘤组织微血管密度可能是其主要作用机制。 【关键词】　胶质瘤；间质内放疗；放射性同位素125I；动物模型；裸鼠；凋亡；电镜形态；　Therapeutic effect of 125 I in the treatment of human glioma nude mice model 　PAN Bao-gen1 ,ZHAO Weng-qing1.2 , li Wen-ling 2, et al . （1，Department of Neurosurgery, Hebei Medical University, shijiazhuang 050031;2，Department of Neurosurgery , Hebei General Hospital, shijiazhuang 050051，china） 【Abstract】　Objective To investigate the efficiency and mechanism of 125 I against the human glioma U251 xenograft . Method While animal models of human malignant glioma were established and 125 I was injected into tumor nodules in vivo，the tumor growth was observed. The ultrastructural change was observed by electron microscopic morphology. The apoptosis of the tumor tissue was detected with in situ end-labelling technique ,their Bax/Bcl-2 expression were detected with flow cytometer. Blood vessel density and expression of VEGF、bFGF were assayed with immunohistochemistry, Results As compared with control group , 125 I can inhibit tumor growth apparently. Observed by electron microscope，It was demonstrated that the tumor cells internally exposed to 125 I displayed nuclear fragmentation，margination of condensed chromatin. Their apoptosis and expression of Bax/Bcl-2 were elevated significantly. Blood vessel density and expression of VEGF、bFGF were decreased significantly. Conclusion 125I has a stronger inhibitory effects on human gliomas .Promoting apoptosis of the neoplastic cell maybe and decreased blood vessel density maybe were the principal mechanisms 【