微藻转基因研究进展.docVIP

工学学士毕业论文 微藻转基因研究进展 冯仕洋 哈尔滨工业大学 2013年6月  生物工程工学学士毕业论文 微藻转基因研究进展 导 师： 郭长禄 申请学位级别： 工学学士 学科、专业： 生物工程 所 在 单 位： 海洋科学与技术学院 答 辩 日 期： 2013年6月 授予学位单位： 哈尔滨工业大学 A Thesis for the Degree of B. Eng Research progress of microalgae genetically modified Candidate： Shiyang Feng Supervisor： Changlu Guo Academic Degree Applied for: forco: for： Bachelor of Engineering Specialty： Biology Engineering Date of Oral Examination： June, 2013 University： ： Harbin Institute of Technology 摘 要 微藻是一类单细胞水生生物，在水产养殖、制药、营养和生物燃料等领域都有着巨大的潜在使用价值。自上世纪报告莱茵衣藻(Chlamydomonas reinhardtii)中存在稳定的基因转化以来，转基因微藻的研究得到了迅速的大规模发展。通过转基因技术，获得新的微藻品种、生产外源性物质、提高产品产率，并最终满足快速发展的市场需求已成为微藻转基因重要的研究目标。 本文从转基因微藻的种类、转基因过程、以及转基因微藻的应用及其展望等方面概述了国内外转基因微藻的研究进展。同时还分析了提高外源基因表达效率和转基因微藻生长速率的各种方法，以利于转基因微藻应用于大规模工业生产。 关键词：微藻；转基因；药物基因；筛选标记；外源基因表达；应用 Abstract Microalgae are a group of unicellular aquatic organisms, having great potential in a number of areas including aquaculture, pharmaceuticals, nutrition, and biofuels. Since last century, the first time stable genetic transformation was reported in Chlamydomonas reinhardtii, genetic transformation of microalgae has been brought to fruition within quite short time. The overarching goal of microalgae biotechnology is to cultivate new species, produce external substances, and enhance the productivity of traditional algal compounds so as to meet the demands of a rapidly growing market. The aim of this review is to synthesize the overall progress on microalgal transformation in the following aspects: the introduction of transgenic microalgae; the process of genetically modified;accomplished biotechnological applications and prospect. At the same time, it analyzed various methods of increasing in the exogenous gene expression efficiency and the growth rates of transgenic microalgae. And this analyzation is good for large-scale industrial production of transgenic microalgae. Key words: Transgenic microalgae; Transform; Therapeutic