海藻胶寡糖的分离制备及电喷雾质谱分析.pdf

150 2011, Vol. 32, No. 02 食品科学 ※分析检测 李 潇，王红敏，黄琳娟，王仲孚 * (西北大学生命科学学院，西北大学糖生物学与糖工程研究中心，陕西省生物技术重点实验室，陕西 西安 710069) 为获得不同聚合度的海藻胶寡糖并对其进行确证，以海藻胶经酶解制得的寡糖产品为原料，对其总糖含 量、糖醛酸含量以及单糖组成等理化指标进行测定，运用硅胶柱层析对寡糖混合物进行分离，以薄层色谱和电喷 雾质谱对所得的寡糖单体进行分析。结果表明：酶解所得到的原料主要为聚合度 2 ～10 的寡居糖醛酸混合物；经 硅胶柱层析分离可获得聚合度 2 ～5 糖的单体。本方法可分离、制备和鉴定海藻胶寡糖。 海藻胶寡糖；分离制备；电喷雾质谱；薄层色谱 Enzymatic Preparation and Electrospray Ionization Mass Spectrometry (ESI-MS) Analysis of Alginate Oligosaccharides LI Xiao ，WANG Hong-min ，HUANG Lin-juan ，WANG Zhong-fu* (Research Center of Glycobiology and Glycotechnology, Shaanxi Provincial Key Laboratory of Biotechnology, College of Life Science, Northwest University, Xi ’an 710069, China) Abstract ：The enzymatic preparation of alginate oligosaccharides with different degrees of plormerization (DPs) from algin was achieved by using 1805 enzyme, an enzyme obtained from the 1805 group of Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Hydrolyzed algin was analyzed for its contents of total sugar and uronic acid and monosaccharide composition, and meanwhile UV spectrometrical analysis at 280 nm was performed to see whether protein is present in it. In addition, the mixture of oligosaccharides was separated by silica column chromatography, and the separated fractions were analyzed by thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). The hydrolysis products of algin were a mixture of oligosaccharides mainly with a DP of 2 －10. Monomers consisting of 2－5 glucose units were substantially separated by silica column chromatography. These investigations will provide some useful references for the preparation, separation and identification of alginate oligosacc