解酒饮保护小鼠急性酒精性肝损伤的研究-中国现代应用药学.pdf

解酒饮保护小鼠急性酒精性肝损伤的研究  庞晓军 1,2 2 ，洪燕坪 (1.钦州市第二人民医院，广西 钦州 535000 ；2.广西医科大学药学院，广西 南宁 530021) 摘要：目的 探讨解酒饮对小鼠急性酒精性肝损伤的保护作用。 方法 将小鼠随机分为正常对照组、联苯双酯组 (11.7 mg·kg1) 、模型对照组和解酒饮低、中、高剂量(6.25 ，12.5，25 g·kg1)组 6 组，每组 10 只，每天 1 次，连续 14 d 1 灌服给药。末次给药后 1 h，除正常对照组外按 12 mL·kg 剂量一次性灌胃给予 56°北京红星二锅头造模。 12 h 后处死 小鼠取血标本和肝组织标本，比较各组的肝脏指数，并对肝组织进行组织形态学检查，测定血清谷丙转氨酶(ALT)、谷草 转氨酶(AST)的活性水平。结果 解酒饮可显著降低酒精诱导的急性肝损伤小鼠的血清 ALT 、AST 水平，可减少肝中脂 肪空泡，减轻炎症表现，急性肝损伤肝脏指数明显改善。结论 解酒饮对小鼠急性酒精性肝损伤有一定的保护作用。 关键词：肝疾病；解酒饮；酒精；急性肝损伤 中图分类号：R285.5 文献标志码：A 文章编号：1007-7693(2017)11-1526-04 DOI: 10.13748/ki.issn1007-7693.2017.11.005 引用本文：庞晓军, 洪燕坪. 解酒饮保护小鼠急性酒精性肝损伤的研究[J]. 中国现代应用药学, 2017, 34(11): 1526-1529. Study on Acute Alcohol Liver Injury Induced by Jiejiu Oral Liquid in Mice 1,2 2 PANG Xiaojun , HONG Yanping (1.Qinzhou Second People’s Hospital, Guangxi, Qinzhou 535000, China; 2.School of Pharmacy, Guangxi Medical University, Nanning 530021, China) ABSTRACT: OBJECTIVE To investigate the protective effect of Jiejiu Oral Liquid on acute alcoholic liver injury in mice. METHODS The mice were randomly divided into 6 groups, 10 rats in each group: normal group, bifendate group (11.7 mg·kg1), model group and low, medium, high dose(6.25, 12.5, 25 g·kg1) group. Mice were administered 1 times a day for 1 single 14 d. Except for the normal control mice were given 56 degrees of Beijing Red Star Erguotou according to 12 mL·kg dose by intragastric administration. Twelve hours later, the mice were killed to take blood samples and liver tissue specimens. Then compared the liver index, histological examination of li