修改读书报告专题讲座.ppt

Results-C/D qRT-PCR Hormonal Stimulation Induces the Formation of Haploid SLCs in Culture FACS C Results-C SGPD-derived co-cultures were supplemented with hormones from days 7–14. Prm1-DsRed-expressing cells were detected on day 10 in the presence of FSH/BPE/T. Scale bars, 100 mm. Results-C A B (A) qRT-PCR analysis of cultures supplemented with hormones as indicated. Relative gene expression levels were normalized to Rps2 and reflect mean ± SD from three independent biological replicates. (B) FACS analysis of DNA content revealing the presence of 14% and 2.6% haploid SLCs only in cultures