玉米须荷叶和蒲公英萃取物护肝功能之研究.doc

PAGE 1 洋蔥萃取物之抗氧化及修復酒精誘導肝細胞損傷作用之探討 Antioxidation and the repairment of ethanol-induced injury in liver cells by onion (Allium cepa L.) extract 研究生：陳宛伶 Wan-Lin Chen 指導教授：古源光、吳明昌 Yuan-Kuang Guu , Ming-Chang Wu 【摘要】 酒品具有特殊的風味，含有維生素、有機酸、多種胺基酸等物質，但攝取過多的酒精會造成肝細胞損傷，進一步導致動脈粥狀硬化症、脂肪肝、肝纖維化及誘發肝癌等症狀。槲皮素屬於類黃酮而廣泛地存在於植物界中，其能夠螯合金屬離子、清除氧自由基、具有抗氧化的特性，根據研究顯示洋蔥所萃取出之槲皮素，具有抗腫瘤、抗過敏、抗病毒及預防心血管病變等功效，對人體健康有益。本研究將利用洋蔥萃取液做為樣品，運用在修復肝細胞經酒精損傷後之修復作用的探討，洋蔥樣品分別有不同洋蔥的品種(紅色、黃色、白色)、不同乾燥處理(冷凍乾燥、60 ℃熱風乾燥)、不同溶液之萃取(70 %酒精、蒸餾水)以及添加不同洋蔥的濃度與細胞反應(0.5、1、5、10、20 mg/mL)等方式來比較其修復細胞的效果。結果顯示，利用0.5 M濃度之酒精培養6小時，做為酒精誘導FL83B細胞最適損傷模式，接者再以冷凍乾燥處理之紅洋蔥於70 %酒精中萃取並添加1 mg/mL於細胞中培養24小時，可達到修復細胞的最佳效果，上述結果是利用WST-1, ALT (Alanine aminotransferase), GGT (γ 關鍵字：酒精、FL83B、洋蔥、肝損傷 【Abstract】 The wine contains special flavor and some nutrients such as vitamins and organic acids. Intake more alcohol would lead to injure the liver cell, such as atherosclerotic, fibrosis, liver cancer etc. Quercetin is one of the flavonoids wildly distributed in the nature and showed the chelated metallic ion, scavenging free radical on the antioxidant characterization, the research showed extract flavonoids of Allium cepa L. can inhibit caner, anti-virus, anti-allergic inflammation and reduce the blood fats etc., it is beneficial to the human health. The Allium cepa L. extract on repair of ethanol damage liver cell will be investigated in this study. The sample from different variety (red, yellow, white), drying treatment (freezing, 60 ℃ hot-air), extraction solvent (70 % alcohol, distiller water) and different concentration (0.5, 1, 5, 10, 20) were treated on the cell. The FL83B cultivated with alcohol for 6 hours with the concentration of 0.5 M has been the alcohol injury cell model. 1 mg/mL 70 % alcohol extraction of red Allium cepa L. with freezing drying was added to cell on 24 hour showed the best on cell repair by The WST-1, ALT (Alanine aminotransferase), GGT (γ-glutamyl transferase) tests to the damaged liver cells. The flavonoids, total phenolic, t