缬草液的大鼠90天喂养试验.doc

缬草液的大鼠90天喂养试验 蒋中仁 刘科亮 徐薇 金伟 卢润生 谢惠萍 [摘要] 目的 评价缬草液对动物的亚慢性毒性。方法 采用经口灌胃法连续90天给予SD大鼠后，观察缬草液对动物血液学指标、血生化指标、脏体比、进食量及食物利用率和组织病理学指标等的影响。结果 雄鼠低剂量组第11周进食量显著降低和高剂量组第13周进食量显著增加（P＜0.05，P＜0.05）；雌鼠中剂量组第10周进食量和高剂量组第11周进食量均显著增加（P＜0.05、P＜0.05），雄鼠中剂量组中期血液学指标中的其它细胞显著升高（P＜0.05），雌鼠中剂量组末期白细胞计数显著降低（P＜0.05）雄鼠低剂量组血糖显著降低（P＜0.05），雌鼠低剂量组尿素氮显著升高（P＜0.05），其余指标未见异常。结论 在本试验条件下，未观察到缬草液明显的毒性反应。 [关键词] 缬草 ；90天喂养；血液学；血生化 ；进食量；食物利用率；脏体比 Ninety Days Rat Feeding Study of Valerian liquid Jiang Zhongren,Liu keliang,Xu Wei,et al (Si Chuan Center For Disease Control And Prevention Abstract Objective To access subchronic toxicity of Valerian liquid. Methods Giving the Valerian liquid to rats by gavage,then evaluate hematology indexs,biochemical indexes organ-body ratios,quantity of food-intake, food availability and pathological effects. Results Both eleven-week’s and thirteen-week’s quantity of food-intake of the low-dosage group were obviousely enhanced in male rats(P＜0.05，P＜0.05）, and ten-week’s quantity of food-intake of the middle-dosage group and eleven-week’s quantity of food-intake of the high-dosage group were obviousely enhanced in female rats(P＜0.05，P＜0.05). There were significant differences in WBC counts of middle-dosage and BUN of low-dosage in female rates(P＜0.05，P＜0.05). The mid-term hematology index of middle-dosage was significantly elevated(P0.05), while blood sugar of low-dosage group were significantly reduced in male rats(P0.05). Conclusion: No subchronic toxicity could be observed. Keywords: Valerian; Ninety Days Feeding Study;hematology indexs;biochemical indexes; quantity of food-intake;food availability; organ-body ratios. 缬草是一种多年生的耐寒开花植物,主要分布在欧洲和亚洲,是近几年被广泛开发利用的具有镇静改善睡眠作用的药食两用植物,目前对其镇静、改善睡眠作用的研究报道比较多[1-2]。将缬草直接加工成粉末后的亚慢性毒性资料已见报道[3]，而醇和水提取物的亚慢性毒性资料国内尚未见报道。为此，我们对缬草的水提物进行了大鼠90天喂养试验，所收集的相关资料为确定人食用的安全剂量提供依据。 1. 材料和方法 1.1材料：缬草液由成都某制药公司生产，成人每日推荐量为10ml/60kg.BW。SD大鼠由四川省医学科学院实验动物研究所提供，动物生产许可证号为SCXK（川）2004-16.SPF级，动物房使用许可证号为SCXK（川）2008-043.屏障系统,温度20-25℃，相对湿度40-70%。CX4型全自动生化分析仪、CA620-Vet型全