高效液相色谱法测定阿昔洛韦眼用凝胶的含量_雷耀龙.pdf

·30 · 2003 23 1 Chin H sp Pharm J, 2003 Jan, V l 23,N .1 雷耀龙,刘艳,李馨儒, 沈传勇　(, 100083) [] 　:建立测定阿昔洛韦眼用凝胶中阿昔洛韦含量的高效液相 谱法。:采用反相Hypersil C18 谱柱(5 μm, 250 mm×4.6 mm)为分离柱;流动相:水-甲醇-冰醋酸(92 ∶8 ∶0.1), 检测波长:252 nm, 以峰面积计算。:线性范围:12 ～32 mg ·L-1, r =0.9999。平均回收率为99.72%, RSD 为0.40%。日内平均RSD 为0.70%, 日间平均RSD 为0.85%。 :本法 简便, 快捷, 灵敏, 可作为该制剂质量控制的有效方法。 [] 　高效液相 谱法;阿昔洛韦;含量测定 [] R927.2　　[] A 　　[] 1001-5213(2003)01-0030-02 Determination of aciclovir eye gel by HPLC LEI Ya -L ng,LIU Yan, LI Xin-Ru, et al(Sch l f Pharmaceutical science, Peking University BeiJing 100083) ABSTRACT:OBJECTIVE　T establish an HPLC meth d f r the determinati n f acicl vir in ey e gel.METHODS　Analytical c l- umn w as Hypersil C (5 μm, 250mm×4.6 mm), The m bile phase w as c mp sed f methan l-water(8 ∶92)with 0.1%glacial acetic 18 acid.The detecti n w avelength was 252 nm and the peak area was calculated.RES LTS　The Calibrati n curve was linear w ithin the c ncentrati n range f 12～32 mg·L-1.The average rec very f acicl vir w as 99.72%, RSD was 0.40%.CONCL SIONS　The meth d is simple, rapid and accurate. KEY WORDS:HPLC;acicl vir;determinati n 　　(acicl vir), , [ 1.2] , 。 ,, , 。 1　 HP 1110 , :GB1365B 1　 ,G 1316A ,G 1311A ( A.　B. 1110 );LD4-2A ( Fig 1　Chr matigram f blank gel and acicl vir c ntr l A.blank　B.c ntr l );( , 630-200001);( 2.0,2.5,3.0,3.5, 4.0 mL, , 200 10324); 25 mL,,20 μL, (, 011120);980NF (BF G drich (Y ) (X ), );(,); Y =57.79X -5 .70, r =0.9999, (,)。 , 12 ～32 mg ·L-1 2 　 。 2.1　　:Heper- 2.3 　　 sil C (5μm,250mm×4.6mm), : ,, 18 --(92 ∶8 ∶0.1), :1 mL ·min-1, 25 mL, 。5 mL , :252 nm, :0.08, 25 ℃, : 4000 r ·min-15 min, 20 μL 。 20 μL。, 2.4 　　 n =11266, 5.691 min。 2.0,3.0, 4 .0 mL, 1。