大承气汤联合嗜酸乳杆菌对重型颅脑损伤小鼠胃肠-第三军医大学学报.doc

大承气汤联合嗜酸乳杆菌对重型颅脑损伤小鼠胃肠动力的影响 付少丽，高英丽，朱丽娜，曾 兢，朱京慈 （400038重庆，第三军医大学护理学院 教研室） [摘要] 目的 探讨大承气汤联合嗜酸乳杆菌对重型颅脑损伤(severe head injury, SHI)小鼠胃肠动力的影响。 方法 将75只雄性C57BL/6小鼠成功制模后按随机数字表法分为5组：假伤组、大承气汤组、嗜酸乳杆菌组、联合用药组(大承气汤+嗜酸乳杆菌)和生理盐水组，每组15只。于伤后4 h干预，干预后1、3、7d取材，检测血浆胃动素(motilin，MTL)含量和小肠推进率，同时采用HE染色法观察脑组织形态学变化。结果 MTL含量：伤后1 d，同假伤组比较，生理盐水组明显降低（P＜0.05），而嗜酸乳杆菌组明显高于生理盐水组（P＜0.05）；伤后3 d，联合用药组均高于生理盐水组和大承气汤组（P＜0.05）；伤后7 d，生理盐水组较假伤组低（P＜0.05），大承气汤组明显高于生理盐水组、嗜酸乳杆菌组和联合用药组（P＜0.05，P＜0.01）。小肠推进率：伤后3 d，生理盐水组明显低于假伤组（P＜0.01）；嗜酸乳杆菌组明显高于生理盐水组和假伤组（P＜0.05，P＜0.01）；大承气汤组和联合用药组均明显高于假伤组和生理盐水组（P＜0.01）。与生理盐水组比较，大承气汤组、嗜酸乳杆菌组和联合用药组脑组织形态均有改善。 结论 大承气汤和嗜酸乳杆菌联合应用可通过调节MTL和肠推进功能有效改善SHI小鼠胃肠动力。 [关键词] 重型颅脑损伤；大承气汤；嗜酸乳杆菌；胃肠动力 [中图法分类号] [文献标志码] A ［基金项目］ 重庆市卫生局中医药科技项目一般项目(2012-2-91) ［通信作者］ 朱京慈，E-mail：zhujingci@163.com [优先出版] Effect of Dachengqi Decoction combined with lactobacillus acidophilus on gastrointestinal motility of mice with severe head injury Fu Shaoli，Gao Yingli，Zhu Lina，Zeng Jing，Zhu Jingci （Nursing school , Third Military Medical University,Chongqing, 400038,China） ［Abstract］ Objective To explore the effects of Dachengqi Decoction(DCQD) combined with lactobacillus acidophilus(LA) on gastrointestinal motility of mice with severe head injury(SHI). Methods 75 C57BL/6 mice of model were built successfully and divided into 5 groups by random number table method: the sham trauma group, DCQD group, LA group, combination group (DCQD+LA), saline group, with 15 in each group. In 4h after SHI intervention, the material were taken at the time points of 1, 3, 7d after intervention. Detecting MTL of blood plasma and small intestinal propulsion rates, using HE staining to observe brain tissue morphology. Results In 1d after SHI, the levels of MTL was lower in saline group than the sham trauma group and LA group(P＜0.05); MTL was higher at 3d after SHI in combination group than saline group and DCQD group (P＜0.05); MTL was lower at 7d after SHI in saline group than the sh