采用固相微萃取-气谱质谱联用技术分析棉花挥发物-应用昆虫学报.doc

请动物所苏建伟帮助审稿。* *基金项目：新疆生产建设兵团博士资金项目（2010JC19） ** E-mail: fhzzky@163.com *** 通讯作者，E-mail: rencixiong@139.com *基金项目：新疆生产建设兵团博士资金项目（ *基金项目：新疆生产建设兵团博士资金项目（2010JC19） ** E-mail: fhzzky@163.com *** 通讯作者，E-mail: rencixiong@139.com 冯宏祖**,王兰,姚永生，熊仁次*** (塔里木大学植物科学学院,新疆阿拉尔 843300) 摘　要: 采用固相微萃取和气质联用技术，对健康棉叶、蚜害棉叶、蚜害棉叶-棉蚜复合物、茉莉酸甲酯处理棉叶以及棉蚜蜜露和棉蚜自身挥发物成分进行了提取和组分分析。共检测出27种挥发性化学物质。健康棉叶、蚜害棉叶、蚜害棉叶-棉蚜复合物、茉莉酸甲酯处理棉叶挥发物中，均含α-蒎烯、β-蒎烯、β-石竹烯、十五烷、十六烷、十七烷、-Cyclohexen-1-ol, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- 7种组份。蚜害棉叶或蚜害棉叶-棉蚜复合物中检测到α-水芹烯、α-石竹烯、α-法尼烯、2,6,10-三甲基十五烷、(E,Z)- 2,6-二甲基-2,4,6-辛三烯、2,6,10,14-四甲基十五烷、蒽、二酚基丙烷8种组份；3-蒈烯、(E)- β-法尼烯只在蚜害棉叶及茉莉酸甲酯处理棉叶中被检测出。在棉蚜及其蜜露中都能检查到壬醛、癸醛、十四烷、十五烷、十六烷、十七烷；而1-甲氧基-4-[(Z)-1-丙烯基]苯、(E)- β-法尼烯、菲只在棉蚜蜜露中被检查到；6,10-二甲基-5,9-十一双烯-2-酮只在棉蚜中被检测到。研究结果为进一步开展棉花、棉蚜挥发物对天敌昆虫的吸引作用研究奠定了基础。 关键词: 棉花；棉蚜；茉莉酸甲酯；棉蚜蜜露；挥发物 Component Analysis analysis of Volatile volatile Compounds compounds Released released from cotton Induced induced by Aphis gossypii and Methyl methyl Jasmonatejasmonate FENG Hong-Zu, WANG Lan, YAO Yong-Sheng, XIONG Ren-ci （College of Plant Science, Tarim University, Alaer 843300, China） Abstract: Volatile compounds of cotton from the leaves uninfected and infested leaves by Aphis gossypii , mixture of aphids and infested leaves, treated leaves by methyl jasmonate, aphids honey dew and its own Volatile volatile compounds were extracted and analyzed by solid-phase microextraction (SPME) and gas chromatography mass spectrometry (GC-MS). It showed that there were altogether 27 species of compounds found. Seven of them, including α-Pinene, β-Pinene, β-Caryophyllene, Pentadecane,, Hexadecane, Heptadecane, -Cyclohexen-1-ol, 1-(1,5-dimethyl-4-hexenyl)-4-methyl- were detected in volatiles of cotton leaves uninfected and infested by Aphis A. gossypii ,. mixture of aphids and infested leaves, and cotton leaves treated by methyl jasmonate. α-Phellandrene, α-Caryophyllene, α-Farnesene, 2,6,10 - trimethyl-pentadecane, (E, Z) - 2,6 - dimethyl -2, 4,6-octatriene, Anthracene, 2,6,10,14-te