生姜根际茎腐病拮抗菌的筛选及鉴定.PDF

微生物学通报 DEC 20, 2010, 37(12): 1767−1770 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 生姜根际茎腐病拮抗菌的筛选及鉴定 1 1,2 1 1 1 1* 王翠翠 杜秉海 姚良同 王璇 徐莹莹 丁延芹 (1. 山东农业大学生命科学学院微生物系 山东 泰安 271018) (2. 山东省农业微生物重点实验室 山东 泰安 271018) 摘 要: 从生姜根际土样分离获得的 86 个细菌分离物中, 通过离体拮抗试验, 筛选出2 株对生姜 茎腐病有良好拮抗效果的细菌, 编号分别为JF24 和JF3 。通过对它们进行形态学观察、生理生化 测定以及16S rRNA序列分析, 初步确定, JF24 为荧光假单胞菌(Pseudomonas fluorescens), JF3 为粪 产碱菌(Alcaligenes faecalis ) 。JF24 和JF3 的16S rRNA 序列的GenBank 登录号分别为FJ999730 和 FJ999731 。 关键词: 生姜根际, 茎腐病, 拮抗, 筛选, 鉴定 Isolation and Identification of Antagonist Bacteria from Ginger Rhizosphere Against Pythium myriotylum Drechsler WANG Cui-Cui1 DU Bing-Hai1,2 YAO Liang-Tong1 WANG Xuan1 XU Ying-Ying1 DING Yan-Qin1* (1. Department of Microbiology, College of Life Science, Shandong Agricultural University, Tai ’an, Shandong 271018, China) (2. Shandong Key Laboratory for Agriculture Microbiology, Tai ’an, Shandong 271018, China) Abstract: Eighty six bacterium isolates were obtained from rhizosphere of ginger. Through antagonistic tests, two antagonistic strains against Pythium myriotylum Drechsler were isolated, which were desig- nated as JF24 and JF3, respectively. Based on the morphological, physiological and biochemical char- acteristics and phylogenetic analysis of 16S rRNA, JF24 and JF3 were identified as Pseudomonas fluo- rescens and Alcaligenes faecali