雪灵芝提取物对H22肝癌荷瘤小鼠免疫功能的影响-肿瘤防治研究.pdf

662 2015 42 7  Cancer Res Prev Treat,2015,Vol.42,No.7 · · 肿瘤防治研究 年第 卷第 期 doi:10.3971/j.issn.1000-8578.2015.07.005 · · 基础研究 H22 雪灵芝提取物对 肝癌荷瘤小鼠免疫功能 的影响 杨歆睿，王刚，吉立宾，王海燕 Effects of Arenaria Kansuensis Aqueous Extract on Immune Function of H22 Tumor- bearing Mice YANG Xinrui, WANG Gang, JI Libin, WANG Haiyan Department of Pathology, Medical College of Qinghai University, Xining 810001, China Corresponding Author: WANG Gang, E-mail: wanggangqh@163.com Abstract: Objective To investigate the effects of Arenaria Kansuensis aqueous extract(AKAE) on the immune function of H22 tumor-bearing mice. Methods Mice bearing H22 solid tumor were randomly divided into five groups: control group, cyclophosphamide(CTX) treatment group and three AKAE treatment groups (high, middle and low dosage). Each group was taken drug through administration by gavage for 10 days. After the mice were killed, the thymus and spleen were weighted respectively to calculate thymus and spleen indexes. The lymphocyte proliferation activity and CTL toxicity were detected by MTT method. The levels of IL-2 and IFN-γ in H22 tumor-bearing mice serum were detected by ELISA. Results After AKAE treatment, the thymus index were (21.39±14.3)mg/g, (17.52±7.7)mg/g, and (30.57±10.2)mg/g in the high, middle and low dosage AKAE group; the spleen index were (38.34±12.7)mg/g, (50.38±29.2)mg/g, and (61.63±35.2)mg/g, respectively. There was significant difference between CTX treatment groups and AKAE treatment groups. There was statistical difference between the low-dose AKAE group and control group(P=0.013). There was statistical difference in the stimulation index(SI) of spleen lymphocyte between high and low-dose AKAE groups(P=0.019