中药饮片蒲黄柴胡和青箱子中微生物污染情况考察-中国药事.pdfVIP

1220 中药饮片蒲黄、柴胡和青箱子中微生物污染情况 考察 刘婷婷，姬俊，牛萌萌，张捷 （青岛市食品药品检验研究院，青岛 266071） 摘要 目的： 方法： 考察 3 种中药饮片蒲黄、柴胡和青箱子中微生物污染状况。 每种饮片选取 10 个批 次，进行需氧菌数、霉菌和酵母菌总数、耐热菌数、耐胆盐革兰阴性菌数、大肠埃希菌、沙门菌等检测。 结果：饮片中需氧菌、霉菌和酵母菌、耐热菌的负载量很高，77% 的饮片检出耐胆盐革兰阴性菌，43% 检出大肠埃希菌，未检出沙门菌。经鉴定，3 种饮片耐热菌主要为芽孢杆菌，耐胆盐革兰阴性菌主要为 结论： γ- 变形菌纲细菌。 中药饮片微生物污染状况严重，饮片的微生物限度标准亟待完善，饮片生产 加工过程控制亟待加强。 关键词： 中药饮片；微生物污染；需氧菌；耐热菌；耐胆盐革兰阴性菌；质量标准 R28 A 1002-7777(2018)09-1220-06 中图分类号： 文献标识码： 文章编号： doi:10.16153/j.1002-7777.2018.09.011 Investigation of Microbial Contamination in Traditional Chinese Medicine Decoction Pieces: Typhae Pollen, Bupleuri Radix and Celosiae Semen Liu Tingting, Ji Jun, Niu Mengmeng, Zhang Jie (Qingdao Institute for Food and Drug Control, Qingdao 266071, China) Abstract Objective: To investigate the microbial contamination in traditional Chinese medicine decoction pieces, such as Typhae Pollen, Bupleuri Radix and Celosiae Semen. Methods: Ten batches of each decoction piece were detected for the numbers of aerobic bacteria, molds and yeasts, thermoduric bacteria, bile-tolerant gram-negative bacteria, Escherchia coli and Salmonella spp and so on. Results: The bioburden of aerobic bacteria, thermoduric bacteria, molds and yeasts in decoction pieces was high. The detection rates for bile-tolerant gram-negative bacteria and Escherchia coli were 77% and 43% respectively. Salmonella spp was not detected. Identification results showed that thermoduric bacteria in three traditional Chinese medicine decoction pieces were mainly Bacillus spp and bile-tolerant gram-negative bacteria were mainly γ-Proteobacteria. Conclusion: The microbial contamination in traditional Chinese medicine decoction pieces is serious. Microbial limit standard needs to be improved urgently and control of the production and processin