毕业论文--酶免法测定乙肝与PCR定量测定乙肝方法学比较.docVIP

本科毕业论文 酶免法测定乙肝与PCR定量测定乙肝方法学比较 PAGE 6 PAGE 3 酶免法测定乙肝与PCR定量测定乙肝方法学比较 ELISA法检测乙肝表面标志物与FQ-PCR①定量检测乙肝病毒DNA的方法学区别及联系 【摘要】：目的 探究酶联免疫法（ELISA）测定乙型肝炎病毒表面标志物（HBsAg、抗-HBs、HBeAg、抗-HBe、抗-HBc简称乙肝两对半）与荧光定量聚合酶链反应（FQ-PCR）定量测定乙肝病毒DNA两种方法学从准确性、特异性、灵敏度等方面的比较【1】。方法 酶联免疫法和FQ-PCR，分别检测500例乙型肝炎患者血清中乙肝五项指标和血清中的乙肝病毒DNA含量。结果 ELISA易观查、适合大规模标本的测定。PCR法测定特异性强、灵敏度更高、准确度高，但仪器设备要求高，易受外因影响，容易存在一定的假阳性或假阴性。适合一些低浓度的HBV患者或ELISA法不能给出明确结果者【2】。结论 ELISA法检测乙肝五项仅可以为临床提供乙肝感染的阴阳性依据，而FQ-PCR法检测乙肝病毒DNA相当灵敏，能详细反映乙肝病毒DNA在体内变化情况，两者在乙肝诊断方面均有相应价值，临床诊断需两者结合，才能达到最优化。 【关键词】ELISA法；荧光定量PCR技术；乙肝血清标志物；乙肝病毒DNA；最优化 The Compare Fluorescence Quantitative PCR Detection of HBV—DNA and ImmunologicalDetection of Hepatitis B by ELISA Abstract: Objective To investigate the enzyme-linked immunosorbent assay (ELISA) assay of hepatitis B virus surface markers (HBsAg, anti--HBs, HBeAg, anti -HBe, anti-hepatitis B two pairs of semi -HBc abbreviation) and fluorescence quantitative polymerase chain reaction (FQ-PCR) HBV DNA Comparison of two methods from accuracy, specificity, sensitivity and other aspects of the quantitative determination. Methods ELISA and FQ-PCR, were detected 500 cases of hepatitis B serum HBV five indicators and serum HBV DNA content. Analysis search results ELISA for measuring large-scale specimens. PCR assay specificity, higher sensitivity, high accuracy, but the equipment requires high vulnerability to external factors, likely there are some false positive or false negative. For some low concentration of HBV patients or ELISA, who can not give a clear result.Conclusion ELISA was used to detect hepatitis B five HBV infection can only provide the basis for clinical yin and yang, and FQ-PCR was used to detect HBV DNA very sensitive, able to reflect in detail HBV DNA changes in the body, both in the diagnosis of hepatitis B have corresponding value, clinical diagnosis must a combination of both in order to achieve optimization. Key words ELISA method; Quantitative PCR; HBV serum markers; hepatitis B virus DNA; optim